Engine knocking poses a significant challenge for downsizing and boosting strategies in spark-ignition (SI) engines. In the event of knock, the unburnt fuel-oxidizer mixture auto-ignites after being compressed by the flame front and piston of an SI engine. Conventional knock is influenced by combustion chemistry and physical properties of the fuel. In this work, we present auto-ignition characteristics of primary reference fuel (PRF75), ethanol, 2,5-dimethylfuran, and their blends in Advanced Fuel Ignition Delay Analyzer (AFIDA). Three different pressures, i.e. 10, 15, and 20 atm and four different temperatures, i.e. 450, 500, 550, and 600 0C have been used as initial conditions. A weak negative temperature coefficient (NTC) behavior has been observed for PRF75 ignition in AFIDA in this work. Moreover, for PRF75, the ignition delay times at low temperatures have been observed to show weaker dependence on pressure in comparison to the high temperature cases. For ethanol and 2,5