Browse Topic: Disinfection

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The onset of the COVID-19 pandemic in early 2020 introduced an unprecedented disruption to global industries, including automotive service and maintenance. As technicians and service shops struggled to balance operational continuity with safety, uncertainty surrounded best practices for servicing potentially dangerous vehicle cabins and air conditioning systems. This paper traces the evolution of these early efforts, from initial confusion and informal guidance to the establishment of the SAE Cabin Disinfection Practices Committee (SAE TEVCDPC) and the eventual publication of SAE J3260 and SAE J3290. It also considers work done by ASHRAE (the American Society of Heating, Refrigerating and Air-Conditioning Engineers), which simultaneously worked on ASHRAE Standard 62.1 and 241. These standards, along with contributions from subject matter experts, formalized the automotive industry’s response to infection control in vehicle environments, integrating scientific understanding with practical service protocols.
Schaeber, StevenMathur, GursaranTaylor, Dwayne
This recommended practice is intended to provide general guidelines for the selection and proper use of technologies and methods intended to minimize the risk of exposure to infection through light-duty vehicle cabin air. It is not intended to include all aspects of cabin air quality, including odor, inorganic particulates, volatile organic compounds (VOCs), etc.
Cabin Disinfection Practices Committee
Sterilization plays a vital role in the use of medical devices. Prior to the 1980s, most medical products were reusable and required sterilization or disinfection between uses. The advance of contagious diseases has raised some concerns over the risks of reusable medical devices, spurring the medical device manufacturing industry to develop disposable, single-use versions of many medical instruments.
This recommended practice is intended to provide general guidelines for the selection and proper use of cleaning and disinfecting product characteristics acceptable for use on vehicle interiors and exterior touch points (cleaning before disinfecting being best practice in general for vehicles, as with other situations), and the effectiveness of the disinfecting products with certain characteristics, as well as indicating the product characteristics that will not cause damage to those surfaces.
Cabin Disinfection Practices Committee
To reduce hospital-associated infections caused by microorganisms, medical devices are typically cleaned and disinfected with chemical disinfectant solutions, including disinfecting wipes. Disinfectant use increased markedly during the COVID-19 pandemic. These disinfectant formulations tend to be comprised of an active ingredient, a solvent or aqueous carrier solution, and additional constituents that enhance the formulation’s properties.
Robots can do many things but they cannot open a door and go through the doorway. Researchers have solved this problem in three-dimensional digital simulations and are building an autonomous robot that can do just that. This simple advance in independence represents a huge leap forward for helper robots that vacuum and disinfect office buildings, airports, and hospitals.
Many of today’s cutting-edge medical technologies utilize advanced sensors to help healthcare specialists provide unprecedented levels of care. Advanced systems and innovative methods allow for improved real-time data and more comprehensive evaluation of the patient’s treatment and overall health; this is true for all facets of medical care — preventive, diagnostic, therapeutic, and overall assessment. With increased use of electronic monitoring technologies also comes the need to protect such sensors and other electronic components from anything that could compromise their performance. Parylene conformal coatings, for example, are often used to protect sensors from environments that can affect their performance over time. Whether they need to withstand potential exposure to simple humidity, pharmaceuticals, or even harsh cleaning and disinfection solutions, long-term sensor performance and reliability remains essential.
FAME is the most common renewable component of conventional automotive diesel. Despite the advantages, biodiesel is more susceptible to oxidative deterioration and due to its chemical composition as well as its higher affinity to water, is considered to be a favorable substrate for microorganisms. On the other hand, apart from biodiesel, alcohols are considered to be promising substitutes to conventional diesel fuel because they can offer higher oxygen concentration leading to better combustion characteristics and lower exhaust emissions. More specifically, n-butanol is a renewable alcohol demonstrating better blending capabilities and properties when it is added to diesel fuel, as its composition is closer to conventional fuel, when compared ethanol to for example. Taking into consideration the alleged disinfectant properties of alcohols, it would be interesting to examine also the microbial stability of blends containing n-butanol in various concentrations. Based on the aforementioned, the aim of this study is to investigate the effect of n-butanol in diesel/ biodiesel blends on fuel quality characteristics (ignition quality, lubricity) while the oxidation and microbial stability is also assessed. Blends of automotive diesel with a commercial FAME up to 20% v/v and n-butanol at concentrations of 5% and 10% v/v were prepared. The microbial stability of diesel/biodiesel/n-butanol blends was assessed and compared to diesel-biodiesel ones by preparing and storing laboratory-scale contaminated microcosms. Overall, ULSD/FAME/n-butanol ternary blends demonstrated high blending stability while density, viscosity, CFPP and sulfur content have not been substantially affected. The poor lubricity of n-butanol and ULSD was compensated by the presence of FAME. N-butanol contributed in increasing the stability - either oxidation or microbial - of the ternary blends compared to the respective binary B7 and B20 blends. Nevertheless, FAME and n-butanol have poor ignition quality characteristics, which resulted in a significant decrease of the DCN of the base fuel.
Dodos, George S.Tsesmeli, Chrysovalanti E.Zahos-Siagos, IraklisTyrovola, TheodoraKaronis, DimitriosZannikos, Fanourios
The fuel supply chain faces challenges associated with microbial contamination symptoms. Microbial growth is an issue usually known to be associated with middle distillate fuels and biodiesel, however, incidents where microbial populations have been isolated from unleaded gasoline storage tanks have also been recently reported. Alcohols are employed as gasoline components and the use of these oxygenates is rising, especially ethanol, which can be a renewable alternative to gasoline, as well. Despite their alleged disinfectant properties, a number of field observations suggests that biodeterioration could be a potential issue in fuel systems handling ethanol-blended gasoline. For this reason, in this study, the effect of alcohols on microbial proliferation in unleaded gasoline fuel was assessed. Ethanol (EtOH), iso-propyl alcohol (IPA) and tert-butyl-alcohol (TBA) were evaluated as examples of alcohols utilized in gasoline as oxygenates. Two different commercial grades of unleaded gasoline were employed in the study, namely a standard (U) and a high octane grade (SU) according to European market nomenclature. The gasoline samples were blended with EtOH, IPA and TBA at various mixing ratios, the resulting blends were contaminated with uncharacterized "bottoms-water" of known microbial activity and the resulting microcosms were stored for a certain period of time. During storage the microbial growth was monitored by utilizing a quantitative microbiological method and alterations in some quality parameters of the stored fuel blends were also examined.
Dodos, George S.Tsesmeli, Chrysovalanti E.Zannikos, Fanourios
O-phthalaldehyde (OPA) is a high-level disinfectant commonly used, for example, for sterilization of heat-sensitive medical instruments; it demonstrates effective microbicidal activity against a wide range of microorganisms (including mycobacteria, gramnegative bacteria, and spores). On the International Space Station (ISS), to achieve thermal control and maintain components at acceptable temperatures, systems that produce waste heat need to have that heat transferred from the ISS to space. To accomplish this, the ISS has an Internal Active Thermal Control System (IATCS) — a water-based system that works in conjunction with the EATCS (External ATCS), an ammoniabased system — to facilitate this heat transfer process.
A concept for a unique zero-g condensing heat exchanger that has an integral ozone-generating capacity has been conceived. This design will contribute to the control of metabolic water vapor in the air, and also provide disinfection of the resultant condensate, and the disinfection of the air stream that flows through the condensing heat exchanger.
Commercial uses for ultraviolet (UV) light are growing, and now a new kind of LED under development at The Ohio State University could lead to more portable and low-cost uses of the technology. The patent-pending LED creates a more precise wavelength of UV light than today’s commercially available UV LEDs, runs at much lower voltages, and is more compact than other experimental methods for creating precise wavelength UV light. The LED could lend itself to applications for chemical detection, disinfection, and UV curing. With significant further development, it might someday be able to provide a source for UV lasers for eye surgery and computer chip manufacture.
Research being done at North Carolina State University will allow the development of energy-efficient LED devices that use ultraviolet (UV) light to kill pathogens such as bacteria and viruses. The technology has a wide array of applications ranging from drinking-water treatment to sterilizing surgical tools.
Two-electron reduction of oxygen to produce hydrogen peroxide is a much researched topic. Most of the work has been done in the production of hydrogen peroxide in basic media, in order to address the needs of the pulp and paper industry. However, peroxides under alkaline conditions show poor stabilities and are not useful in disinfection applications. There is a need to design electrocatalysts that are stable and provide good current and energy efficiencies to produce hydrogen peroxide under acidic conditions.
A Pilot Scale System for Low Temperature Solid Waste Oxidation and Recovery of Water2009-01-23657/12/2009
In February 2004 NASA released “The Vision for Space Exploration.” The goals outlined in this document include extending the human presence in the solar system, culminating in the exploration of Mars. A key requirement for this effort is to identify a safe and effective method to process waste. Methods currently under consideration include incineration, microbial oxidation, pyrolysis, drying, and compaction. Although each has advantages, no single method has yet been developed that is safe, recovers valuable resources including oxygen and water, and has low energy and space requirements. Thus, the objective of this work was to develop a low temperature oxidation process to convert waste cleanly and rapidly to carbon dioxide and water. TDA and NASA Ames Research Center have developed a pilot scale low temperature ozone oxidation system to convert organic waste to CO2 and H2O. The system not only extracts water from the waste, but it also recovers the water produced from the ozone oxidation of the solid waste that can be utilized by the crew. During treatment the system also disinfects any residue remaining in the reactor. Experiments were conducted with solid wastes to demonstrate the process at large scale, quantify system level performance and identify the parameters that will optimize system design to full scale. The waste loading scales with the reactor size and the oxidation rate primarily scales with the ozone flow and its utilization through the reactor. At present, the average waste oxidation rate of a fecal simulant is 10.5 g/h, which can be increased to 36 g/h by replacing the ozone generator with a higher output unit. These data, along with current waste generation rate models, indicate that all of the waste from a single crew member in one day can be processed in a vessel that is 9.5 liters (2.5 gallons) for long term missions.
Nabity, James A.Andersen, Erik W.Engel, Jeffrey R.Wickham, David T.Fisher, John W.
This report describes the design, assembly, and testing of a modified, re-circulating drip flow reactor to quantify the electrical, optical, and thermal performance of solid-state ultraviolet (UV) lighting and semi-conducting photocatalyst for potable water disinfection by advanced oxidation processes. The reactor test assembly incorporates high-output UV-A Light Emitting Diodes (LEDs) with active thermal control to reject heat and generate reactive oxygen species from immobilized titanium dioxide attached to borosilicate glass in the laminar flow stream. Compared with UV-excimer and UV-mercury arc lamps, the UV-A LED system demonstrated excellent thermal stability and good electrical and optical performance.
Rodriguez, Nadia SilvestrySoler, Robert R.Koss, Lawrence L.Maxik, FredSchuerger, Andrew C.Roberts, Michael S.
ISS IATCS Coolant Loop Biocide Implementation2008-01-21596/29/2008
The proliferation and growth of microorganisms in the Internal Active Thermal Control System (IATCS) aboard the International Space Station (ISS) has been of significant concern since 2001. Initial testing and assessments of biocides to determine bacterial disinfection capability, material compatibility, stability (rate of oxidative degradation and identification of degradation products), solubility, application methodology, impact on coolant toxicity hazard level, and impact on environmental control and life support systems identified a prioritized list of acceptable biocidal agents including glutaraldehyde, ortho-phthalaldehyde (OPA), and methyl isothiazolone. Glutaraldehyde at greater than 25 ppm was eliminated due to NASA concerns with safety and toxicity and methyl isothiazolone was eliminated from further consideration due to ineffectiveness against biofilms and toxicity at higher concentrations. Therefore, OPA, an aromatic dialdehyde compound with the formula C6H4(CHO)2 was selected as the optimum antimicrobial for use in the ISS IATCS and was approved for use at concentrations <109 ppm (1). A key aspect of the biocide use on the ISS was the method in which the biocide would be delivered to the coolant system. A non-intrusive implementation technique was required in order to add the OPA to the IATCS. The method developed utilized the current NiRA (Nickel Removal Assembly) packed bed hardware including a 2 liter canister, flex hoses and quick disconnects. An immobilization of the OPA to an inert resin substrate was selected as the process to package for delivery. Development of the immobilization procedure involved determining proper OPA loading density to the resin material as well as proper placement of the biocide loaded resin in the packed bed to meet requirements for biocide elution profile and final coolant concentration. Significant ground testing at both the 1/10th and full scale levels demonstrated that adequate concentrations could be added to the IATCS in the required time envelope while maintaining compliance with system requirements and safety. In addition to the development of the OPA delivery resin material, means to adequately determine on-orbit aqueous OPA concentrations were also investigated. Since the frequency of on-orbit sample return is usually greater than 3 to 6 months for laboratory verification of biocide concentration, on-orbit determination of biocide concentration was deemed imperative. A partnership between Hamilton Sundstrand and Branan Medical Corporation was undertaken to investigate the feasibility of manufacturing OPA colorimetric concentration test strips. Test strip development for OPA concentrations from 25 ppm to 200 ppm was achieved and successfully manufactured for on-orbit use. The OPA delivery resin and test strips were delivered to the ISS on flight STS-120 and subsequently used during that mission. This paper reports on all development activities associated with the OPA delivery resin and test strips as well as the successful implementation of OPA to the ISS IATCS.
Rector, TonySteele, JohnWilson, Mark
Assessment of Silver Based Disinfection Technology for CEV and Future US Spacecraft2007-01-32587/9/2007
Silver biocide offers a potential advantage over iodine, the current state-of-the-art in US spacecraft disinfection technology, in that silver can be safely consumed by the crew. As such, silver may reduce the overall complexity and mass of future spacecraft potable water systems, particularly those used to support long duration missions. A primary technology gap identified for the use of silver biocide is one of material compatibility. Wetted materials of construction are required to be selected such that silver ion concentrations can be maintained at biocidally effective levels. Preliminary data on silver biocide depletion rates in heritage spacecraft potable water system wetted materials of construction has been gathered as part of a multi-phase test project aimed at the characterization of silver based biocide technology through: development of preferred materials lists, investigation of silver biocide forms and delivery methods, down-selection of silver biocide technologies, and integrated testing. Preliminary test data has shown a 10 to 20% loss in silver ion concentration per day for acid passivated Nitronic 40 tubing tested at surface area to volume (S/V) ratios approximating 4.59 cm-1. The Nitronic tubes were tested both with and without biocide pretreatment. Silver biocide depletion was also observed at approximately 0.1% per day for the first 35 days of exposure to acid passivated Inconel 718 coupons tested at low S/V ratios, ∼ 0.14 cm-1. Surface analysis by scanning electron microscopy (SEM) suggested deposition of silver metal on both test materials. SEM analysis also provided evidence of potential variability in the passivation process when using materials tested in a tube format. These preliminary results are presented and discussed herein, along with the current project status.
Callahan, Michael R.Adam, Niklas M.Roberts, Michael S.Garland, Jay L.Sager, John C.Pickering, Karen D.
Assessment of Silver Based Disinfection Technology for CEV and Future US Spacecraft: Microbial Efficacy2007-01-31427/9/2007
This work describes the microbiological assessment and materials compatibility of a silver-based biocide as an alternative to iodine for the Crew Exploration Vehicle (CEV) and future spacecraft potable water systems. In addition to physical and operational anti-microbial counter-measures, the prevention of microbial growth, biofilm formation, and microbiologically induced corrosion in water distribution and storage systems requires maintenance of a biologically-effective, residual biocide concentration in solution and on the wetted surfaces of the system. Because of the potential for biocide depletion in water distribution systems and the development of acquired biocide resistance within microbial populations, even sterile water with residual biocide may, over time, support the growth and/or proliferation of bacteria that pose a risk to crew health and environmental systems. This work reports the efficacy of [500 ppb] silver fluoride in potable water as a biocide against each of five bacterial species previously isolated from spacecraft and the materials compatibility of the biocide with each of three heritage materials used in US spacecraft potable water systems (i.e., Inconel 718, 21-6-9 Stainless Steel (aka Nitronic 40), and 316 Stainless Steel). Silver concentration, total microbial load and materials analysis of submerged metal coupons were assessed at six time-points over the course of replicated, short-duration 21-day experiments using two surface area-to-volume coupon ratios representing those in the shuttle potable water bellows tank and water distribution system. These experiments are part of a multi-phase evaluation of silver-based biocide technology for CEV applications through development of preferred materials lists, investigation of silver biocide forms and delivery methods, down-selection of silver biocide technologies, and integrated system testing.
Roberts, Michael S.Hummerick, Mary E.Edney, Sharon L.Bisbee, Patricia A.Callahan, Michael R.Loucks,, SandyPickering, Karen D.Sager, John C.
A prototype of an electroporation system for sterilizing wastewater or drinking water has been developed. In electroporation, applied electric fields cause transient and/or permanent changes in the porosities of living cells. Electroporation at lower field strengths can be exploited to increase the efficiency of chemical disinfection (as in chlorination). Electroporation at higher field strengths is capable of inactivating and even killing bacteria and other pathogens, without use of chemicals. Hence, electroporation is at least a partial alternative to chlorination.
Preparation of Nanostructured Photocatalytic TiO 2 Films and Membranes Using Sol-Gel Methods Modified with Surfactant Micelles for Wastewater Treatment and Reuse in Space2005-01-29927/11/2005
This study describes the application of novel chemistry methods for the fabrication of robust nanostructured TiO2 photocatalytic films and membranes for the development of efficient wastewater treatment and reuse systems for advanced life support in space missions. These films and membranes perform several functions, including water purification (i.e., destruction of organic contaminants, filtration) and water disinfection. Nanocrystalline TiO2 thin films and membranes, and the corresponding particles have been synthesized from a titania sol modified with nonionic long chain surfactants used as pore directing agents. The prepared materials were characterized to reveal material crystal structure, morphology, nanostructure, elemental composition, and other characteristics, and evaluated for their adsorptive properties and photocatalytic activity to destroy organic contaminants in water. The prepared TiO2/Al2O3 composite membranes showed promising results with respect to water permeability and organic retention. These TiO2 photocatalysts will be crucial components for the fabrication of integrated photocatalytic reactors and membranes for the low-temperature production of clean potable and hygienic water in space missions.
Choi, HyeokDionysiou, Dionysios D.Stathatos, Elias
Photocatalytic Oxidation of Selected Organic Contaminants and Inactivation of Microorganisms in a Continuous Flow Reactor Packed with Titania-Doped Silica2005-01-29917/11/2005
Two annular continuous flow reactors with nominal volumes of 400 mL and 150 mL were packed with silica gel pellets that were doped with titania (TiO2) (12 wt%). The reactors were configured with UV lamps in the center of the reactors. SOC oxidation experiments were performed in a single-pass mode with bicarbonate ions present and in a low dissolved oxygen environment. SOC concentrations decreased (ranging from 40% to 95%) without bicarbonate present. These removal efficiencies were not affected by moderate bicarbonate concentrations (up to 200 mg/L as NaHCO3) or low dissolved oxygen levels (2 mg/L). Microbial experiments were performed for the inactivation of selected viruses and bacteria. The log [N0/N] values resulting from two hours of 254-nm UV irradiation for the bacteriophages ФX-174, PRD-1, and MS-2 were 1.67, 1.43, and 1.65, respectively. The log [N0/N] values resulting from two hours of 254-nm UV irradiation for the bacteria Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa were 1.70, 1.40, and 1.62, respectively. In some instances experiments performed without pellets in the reactor produced greater inactivation, but in all experiments inactivation was below drinking water disinfection requirements.
Garton, M. JoanneChadik, Paul A.Mazyck, David W.Farrah, Samuel R.
Modeling and Design of an Ultraviolet Water Disinfection System2005-01-30617/11/2005
The goal of this research is to design an ultraviolet (UV) disinfection reactor that will inactivate pathogenic microorganisms present in the wastewater generated during long-term space missions, such that complete reuse (i.e., direct potabilization) can be accomplished. This design must ensure microbial inactivation efficacy, as well as minimize volume, mass, power and maintenance requirements. The means to achieve this design goal is a numerical modeling tool developed in this research, which is based on Computational Fluid Dynamics (CFD), UV radiation intensity field models and microbial inactivation kinetics. The inputs to this numerical model are the desired reactor size and geometry, the inlet velocity and boundary conditions, the UV lamp output power and radiation intensity profile, as well as the characteristics of the aqueous media. The outputs of the model are the UV dose distribution delivered to the microorganisms traversing the reactor and the degree of microbial inactivation achieved. Based on these outputs, the performance of the UV reactor can be assessed for the entire range of practical operating conditions. The validity of the numerical model was assessed with biodosimetry experiments employing Bacillus subtilis spores as the target microorganism and a commercially available UV disinfection reactor. The numerical model is used to investigate alternative UV reactor geometries which can be incorporated into an Advanced Life Support (ALS) water purification system for long-term space missions. The simulation input flow rate is based on the daily water output from six crewmembers and the biomass production chamber, which is included in the ALS closed-loop water system. UV reactor designs are evaluated based on dual criteria: process efficiency expressed as the degree of achieved microbial inactivation, as predicted by the numerical model, and Equivalent System Mass (ESM) values.
Naunovic, ZoranaShen, ChengyueLyn, Dennis A.Blatchley, Ernest R.
Quality of ATV Potable Water for ISS Crew Consumption2004-01-24917/19/2004
Potable water is undoubtedly one of the most critical resources for the International Space Station (ISS) crews. The amount and quality of this resource, mainly provided to the ISS by the Space Shuttle and Progress, and in the near future by logistic vehicles Automated Transport Vehicle (ATV) and HTV, must be compatible with the crew consumption needs and health-related requirements. For this purpose, potable water must satisfy very stringent quality requirements from chemical and bacteriological point of view. The definition of such requirements, resulting from medical studies, lessons learned, technical constraints, is reached in agreement among ISS International Partners. Two different quality standards are defined, one for the ISS Russian Segment, the other for the US Segment and other International Partners. The ATV, a program under European Space Agency (ESA) contract and EADS Space Transportation (EST) prime contractor-ship, is the only logistic vehicle requested to transport and deliver potable water to ISS according to both quality standards. Significant efforts have been spent in Alenia Spazio, responsible for the ATV Integrated Cargo Carrier, to define all the activities necessary to accomplish this task. The main aspects under consideration have been: selection of materials in contact with water, identification of suitable potable water sources, water preparation and disinfection, and pre-conditioning of equipment. This paper focuses on preparation and quality of potable water as obtained in dedicated ground facilities. The quality and stability of source water are an essential pre-requisite to attain the required standards. Disinfection techniques using colloidal silver and iodine are discussed, and their implementation in the ATV Water Preparation Facility at Società Metropolitana Acque Torino (SMAT) premises is presented. The results of chemical and micro-biological analyses performed on potable water batches treated with the defined techniques show that the requirements are fully satisfied.
Lobascio, CesareBruno, GiosuèGrizzaffi, LuciaMeucci, LorenzaFungi, MartinoGiacosa, Donatella
Synergistic UV-Ozone Effects on the Treatment of Pathogens in Secondary Effluent2003-01-25637/7/2003
Ozone and ultraviolet (UV) light have been shown to be effective in treating emerging pathogens that are not affected by the current standard treatment of chlorine. The hypothesis of this study was that a combined treatment of UV light and ozone would result in greater pathogen reduction than a large dose of either one individually. In the one-way studies for ozone and UV light, the ozone treatments took at least twice as long as the UV treatments to achieve the same extent of reduction in the microbial population. Specifically, 60 min for ozone and 30 min for UV light for a 4-log reduction in Heterotrophic plate counts (HPC's); 10 min for ozone and 3 min for UV light for a 3-log reduction in total coliforms and fecal coliforms; and, 6 min for ozone and 3 min for UV light for a 2-log reduction in coliphage. The ozone treatments, however, unlike the UV treatments, resulted in no positive re-growth in bacteria after treatment. Ozone and UV light acting simultaneously exhibited not merely additive, but synergistic effects, in reducing the microbial populations, with the exception of when low levels (2 min UV and 1 min ozone) of both factors were used. Specifically, the following treatment combinations yielded the maximum microbial reductions: low level of UV (1 min) and high level of ozone (4 min) for HPC's; high level of UV (2 min) and high level of ozone (4 min) for total Coliforms and fecal coliforms; and either one of the foregoing treatments for the overall microbial population. The synergistic effects between UV light and ozone allowed for lower UV and ozone levels to achieve the same extent of microbial reduction achieved by either factor acting separately. Thus, 2 min of ozone and 2 min of UV light applied simultaneously was just as effective as 6-8 min of ozone only or 2.5 min of UV light only. This synergistic effect should lead to reduced energy consumption while maintaining the level of disinfection required. If UV light and ozone were to be applied successively, the most effective sequence of application would be ozone first followed by UV light.
Kuwahara, S.Cuello, J.Reynolds, K.Gerba, C.
A $90 million AUS (Australian dollar) upgrade ($49 million USD) to the Cronulla Sewage Treatment Plant in Southern Sydney, Australia, was undertaken to meet the requirements of a growing population and to add advanced sewage treatment processes including ultraviolet disinfection. During the design phase, a cost-effective engineering solution needed to be developed for a new aeration system that provides air to the biological reactors. Design, Detail and Development, a division of Blenray Pty. Ltd., used ALGOR's piping design and analysis software, PipePak, to analyze modifications to the aeration system to ensure that the new design could withstand expected thermal strains. The initial design contained numerous, expensive stainless steel bellows to account for thermal expansion and contraction. The final design of the system replaced bellows with spiral-wound stainless steel, which saved $150,000 AUS ($81,800 USD) and helped to keep the project on budget. The system has been installed and is functioning successfully.
Long-term space flight missions will require high quality water to lessen the risk of crew infections and system deterioration. In water systems on earth, biofilms contribute to loss of water quality, causing corrosion, increased flow resistance and reduced heat transfer. Some bacteria grow more rapidly and become less susceptible to antimicrobial agents under conditions of microgravity, and humans may have weakened immunity with prolonged space flight. This study aimed to determine the effects of spaceflight and microgravity on biofilm formation by Burkholderia cepacia in water and microbial control by iodine. The results showed that B. cepacia formed biofilms when incubated in microgravity and in ground controls. Compared to rich medium or water, biofilms developed at similar densities in iodinated water. Thus, disinfection and maintenance of spacecraft water systems should take into account the difficulties of controlling attached bacteria, which are commonly encountered in earth-based systems.
Pyle, Barry H.Broadaway, Susan C.McFeters, Gordon A.
Reduction in the Iodine Content of Shuttle Drinking Water: Lessons Learned1999-01-21177/12/1999
Iodine is the disinfectant used in U.S. spacecraft potable water systems. Recent long-term testing on human subjects has raised concerns about excessive iodine consumption. Efforts to reduce iodine consumption by Shuttle crews were initiated on STS-87, using hardware originally designed to deiodinate Shuttle water prior to transfer to the Mir Space Station. This hardware has several negative aspects when used for Shuttle galley operations, and efforts to develop a practical alternative were initiated under a compressed development schedule. The alternative Low Iodine Residual System (LIRS) was flown as a Detailed Test Objective on STS-95. On-orbit, the LIRS imparted an adverse taste to the water due to the presence of trialkylamines that had not been detected during development and certification testing. A post-flight investigation revealed that the trialkylamines were released during gamma sterilization of the LIRS resin materials. The LIRS effluent water quality was not completely tested after gamma sterilization as previous experience and limited testing suggested that gamma irradiation would not degrade the resins. In addition, concerns about microbial contamination ruled out testing of the flight hardware following sterilization. The lessons learned from the experience were that experiments with the potential to impact the Shuttle life support system should be classified as critical hardware, and that certification testing of all hardware parameters must be carried out in the final flight configuration.
Wiederhoeft, Curt J.Schultz, John R.Michalek, William F.Sauer, Richard L.
Rapid Enumeration of Active Bacteria in Water After Disinfection9817617/13/1998
Drinking water aboard spacecraft and on earth must be monitored to ensure that harmful bacteria are absent. NASA needs rapid methods for this purpose, to avoid possible launch delays and limit potential water-related health risks aboard spacecraft on orbit. Determination of bacterial viability after exposure to disinfection has significant health importance since oxidatively injured pathogenic bacteria have been shown to retain their virulence. This problem is compounded by the observation that injured bacteria are recovered at significantly lower frequencies using standard agar plate assays, leading to an underestimation of infection risks. Escherichia coli O157:H7 was exposed to 0.5 ppm free chlorine, retained on membrane filters and tested for physiological activity using a variety of assays. Physiological activity was assessed by detection of membrane potential [rhodamine 123 (Rh123)], substrate responsiveness [direct viable counts (DVC)], respiratory activity [5-cyano-2,3-di-4-tolyltetrazolium chloride (CTC)] and the LIVE/DEAD BacLight Viability kit. Loss of physiological activity was determined by detection of the absence of membrane potential {bis-(1,3-dibutylbarbituric acid) trimethine oxonol [DiBAC4(3)]} and the LIVE/DEAD BacLight Viability kit. Injury and total culturable counts were determined using TLY, TLYD and R2A agars. Total cell counts were determined using DAPI. CTC detected 20.6 to 42.7% more active bacteria than the other physiological assays and 19.0% more than R2A agar prior to disinfection. Following disinfection, CTC detected 16 to 35.4% and 42% more active bacteria than the other assays and R2A agar, respectively. When DiBAC4(3) was used in conjunction with CTC, 102% and 103% of the total cell count was accounted for prior to and following disinfection, respectively. The LIVE/DEAD BacLight Viability kit accounted for 109 and 107% of the total cell count under identical conditions. A method is described where CTC, DiBAC4(3) and a tetramethyl rhodamine isothiocyanate (TRITC) labeled fluorescent antibody (FAb) are used concurrently permitting rapid evaluation of levels of activity (estimates of viability) and total cell counts on a single membrane filter. Immunomagnetic separation (IMS) has been used to enhance sensitivity. Superparamagnetic beads coated with an antibody which reacts with O157 cells were added to water samples, and a magnet was used to separate the beads with bacteria attached to them. Following CTC incubation, filtration and staining with an O157-specific fluorescein isothiocyanate (FITC) labeled antibody, the cells were enumerated by fluorescence microscopy or a rapid solid-phase laser scanner (Scan RDI, Chemunex). Resultant enumeration was closely related to plate counts, and detection of as few as one E. coli O157:H7 cell was possible with the laser scanning method. Methods such as those described are candidates for future spacecraft use by NASA, and will also be useful in the microbiological examination of water and food on earth. A military application is the detection of pathogenic bacteria in combat zones. The prototype CTC/FAb technique for E. coli O157:H7 detection can be applied to the enumeration of any bacterium for which a suitable antibody can be produced. The procedure can be completed with 6-8 hours, and is much more sensitive than existing procedures. Ultimately, techniques like these which do not require cultivation of bacteria are likely to replace existing culture methods because of their rapidity, sensitivity, and specificity.
Lisle, John T.Pyle, Barry H.Broadaway, Susan C.McFeters, Gordon A.
Depletion of Biocidal Iodine in a Stainless Steel Water System9413916/1/1994
Iodine depletion in a simulated water storage tank and distribution system was examined to support a larger research program aimed at developing disinfection methods for spacecraft potable water systems. The main objective of this study was to determine the rate of iodine depletion with respect to the surface area of the stainless steel components contacting iodinated water. Two model configurations were tested. The first, representing a storage and distribution system, consisted of a stainless steel bellows tank, a coil of stainless steel tubing and valves to isolate the components. The second represented segments of a water distribution system and consisted of eight individual lengths of 21-6-9 stainless tubing similar to that used in the Shuttle Orbiter. The tubing has a relatively high and constant surface area to volume ratio (S/V) and the bellows tank a lower and variable S/V. The test apparatus was cleaned and filled with a 10 mg/L iodine solution prepared with distilled-deionized water. Sampling from the tank, coil, and the series of tubes was performed periodically, and the major iodine species were monitored to determine the rate of decay of iodine and its fate in the system. Results of this study show that the iodine demand of stainless steel was significant. Decay rates were higher than anticipated in tubing that had not been previously exposed to iodine. The iodine (I2) concentration in the tank contents decreased to less than 1 mg/L in 10 weeks, whereas none was detected in water from the tubes after 1 week. Also, trace metals were measured in the samples by graphite furnace atomic absorption spectrometry and found to increase with contact time. A subsequent attempt to passivate the inside surface of the tubing via a 4-hour soak with 30 mg/L iodinated water reduced the rate but did not eliminate iodine loss. Ramifications of these findings to the current Shuttle potable water system and the design of spacecraft water systems in general are discussed.
Mudgett, Paul D.Flanagan, David T.Schultz, John R.Sauer, Richard L.
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