Electric wheel dump trucks are mainly used in open pit mines, where the working environment is very harsh and the driver's continuous working time is extremely long, therefore, the ride comfort of the truck is pretty important. This paper evaluates and optimizes the ride comfort, according to ISO2631, and Chinese standard GB/T4970-1996 and Chinese standard QC/T76.8-1993, while the ride comfort test had been done in a open pit mine. After the test data was analyzed, the results showed that the ride comfort of this truck needs to be improved and optimized. The multi-body system dynamic model was built in MATLAB/SIMULINK for this dump truck, to simulate the realistic working condition using a D-class road, which was reconstructed according to ISO/DIS8608 and Chinese standard GB7031-86, while the simulation results were in coincidence with the test ones. Finally, by terms of the nonlinear characteristics of the hydro-pneumatic suspension, the dump truck ride comfort was optimized using the genetic algorithm, while the results showed that the vehicle ride comfort has been improved significantly.