In allusion to the dynamic leak phenomenon caused by the fuel sloshing in the dynamic driving process of vehicle, the dynamic leak detection method was proposed based on the analysis of the dynamic leak mechanism and influencing factors. The six degree of freedom platform was used to simulate the gesture change of fuel tank during driving process. The influence of fuel tank operating conditions and different design styles on dynamic leak were mainly studied. Fiber-optic gyroscope was used to acquire the operating conditions of the fuel tank during driving process in five regions of China: Wuxi Mountain in Anhui, Hongjing Road in Beijing, Jing’anyi in Yunnan, Wuzhi Mountain in Hainan, Gele Mountain in Chongqing. Signal acquired by fiber-optic gyroscope includes the acceleration of the vehicle in the three directions of forward and backward, left and right, up and down as well as the rotation angles around these three directions. The displacement signal after integral reconstruction and angle signal jointly constitutes the operating conditions of fuel tank in different regions. As a comparison, South Mountain operating condition in the United States was also used for analysis. Small type, flat type, L type, saddle type and longitudinal strip type fuel tanks were selected as research objects. The six degree of freedom platform was used to simulate the gesture change of fuel tank during driving process. The result shows that the operating condition of South Mountain in the United States is quite different from those of five regions in China. Among the fuel tank operating conditions in China, the total dynamic leak amount of Wuxi Mountain operating condition in Anhui is the largest, and the leak rate result shows that L type fuel tanks are more prone to dynamic leak. Finally, according to the test results, the best dynamic leak detection operating conditions for fuel tanks with different design styles are proposed.