The Mellin non-uniformly distributed moving blade method was adopted to conduct CFD numerical analysis and sample experimental tests on the axial-flow turbines before and after optimization using the uniformly distributed and non-uniformly distributed design methods, respectively. In the original design, five blades were evenly distributed in the 360° circumferential direction, and the non-uniformly distributed angles were 46°, 102°, 46°, 83°, and 83°. CFD numerical analysis shows that due to the low rotational speed of the turbine and the absence of a sealing structure at the blade tip, factors such as tip noise leakage and backflow have little impact, and the flow field pulsation is mainly caused by the blades themselves. The non-uniformly distributed design can significantly enhance the work-doing capacity of the blades. At 90% of the blade height, the torque can be increased by up to 60%, but at the same time, the axial force on the blades also increases accordingly. Near 80% - 90% of the blade height, the axial force increases by 33%. The flow rate performance of the non-uniformly distributed design is slightly inferior to that of the uniformly distributed design, but the overall noise is better than that of the uniformly distributed design, with maximum optimization of 0.48 dB (A); the maximum values of the first three orders of discrete noise are significantly improved, with a maximum improvement of 0.75 dB (A), and the discrete noise orders of the non-uniformly distributed turbine can avoid blade - related factors and disperse the energy to nearby orders.