This experimental investigation was carried out for 150 hours endurance test in the time interval of 50 hours each, with test fuel neat diesel, Calophyllum oil biodiesel blend B25 and water in Calophyllum (WIC) 10 vol. % to blend B25-WIC in a single cylinder diesel engine. The experiment was conducted at constant conditions of engine load 3.0 kW and speed of 1500 rpm. The impact of 150 hours endurance test on injector nozzle deposition and engine parameters like Brake specific fuel consumption, Brake thermal efficiency, Brake specific energy consumption, Exhaust gas temperature, Carbon dioxide, Carbon monoxide, Hydrocarbon, and Oxides of nitrogen were investigated. The findings revealed that water inclusion (10 vol. %) in Calophyllum oil biodiesel blend B25-WIC has a positive impact on diesel engine performance and emission characteristics as compared to biodiesel blends without water inclusion. Gummy and heavy deposition accumulation was observed with biodiesel blend B25 which was higher by 12% than B25-WIC with thin and dry deposition. Engine performance parameters when compared to neat diesel were reported as; BSFC higher for B25 and B25-WIC by 5.85 and 3.99% respectively. The average reduction in BSEC with B25 was 0.025% and by 6.55% with B25-WIC. And BTH was observed to be reduced by 18.14% with B25 and by 10.01% with B25-WIC. Exhaust emissions parameter observed against neat diesel as; CO2, CO, HC, and NOx shows reduction for B25 by 63.91%, 15.14%, 7.94%, and 2.38% respectively. And for B25-WIC by 52.36%, 27.19%, 18.14%, and 4.56%respectively.