Due to the energy safety and environment protection, increase the percentage of biodiesel blend has become one of world wide strategies. In the past research, using biodiesel would affect the engine performance and increase the exhaust emission. Fortunately, these problems can be solved through the rapidly development of engine control technologies and lightweight structure design. However, the consideration of light/downsizing engine design with the same power has brought out much combustion noise. According to the higher and higher proportion has been widely used over the world. There was less researches focus on the different blending biodiesel impact on combustion noise. The combustion noise correspond to different blending biodiesel (D100,B5,B8,B20,B40,B100) which made form waste cooking oil has been discussion in this study. The experimental by using engine which meet EURO-4 was designed to caught spectrum of the combustion noise via transient window which under the constant engine speed of 1500rpm, 2000rpm, 2500rpm, with different torque at 30%, 50% and 70% of each speed, respectively. The overall result shows that different proportion blending of biodiesel related to combustion noise directly and the combustion noise raises 0.5 dB (ref. 1 bar) by using 20% biodiesel blend. In this study the influence trend curve map of engine speed and torque values correspond to combustion noise have been gotten. It would help us to get a win-win-win situation and to optimize the objective function of engine control model in the future.