As a crucial part of national strategic resources, petroleum is an important basic material for economic development. However, during the storage, loading and unloading, and transportation of bulk liquid petroleum products, unavoidable natural losses occur due to factors such as process technology and equipment. Therefore, studying the natural loss of liquid petroleum during storage and transportation, and adopting effective countermeasures to minimize the natural loss of liquid petroleum, has become a topic of focus in various fields. This paper uses the “Loss of Bulk Liquid Petroleum Products” approved in 1989 as the analysis standard to explore the natural loss of highway oil transportation, conduct statistical test analysis on oil data such as oil collection registration forms, and propose conclusions and suggestions, thereby providing a reference for the revision of oil loss standards. The experimental results show that the overall oil data meets the national standard for natural loss of bulk liquid petroleum products, and suggests revising three standards, indicating that the standard setting is relatively scientific and reasonable.