Gaseous and particulate emissions from heavy-duty vehicles are affected by fuel types, vehicle/engine parameters, driving characteristics, and environmental conditions. Transient chassis tests were conducted on twenty-six heavy-duty vehicles fueled with methanol, compressed natural gas (CNG), #1 diesel, and #2 diesel, using West Virginia University (WVU) Transportable Heavy-Duty Vehicle Emissions Testing Laboratory. The vehicles were operated on the central business district (CBD) testing cycle, and regulated emissions of carbon monoxide (CO), total hydrocarbon (HC), nitrogen oxides (NOx), and particulate matter (PM) were measured. Comparisons of regulated emissions results revealed that the vehicles powered on methanol and CNG produced much lower particulate emissions than the conventionally fueled vehicles. The emissions of CO, NOx, and HC depend on the fuels as well as many other parameters such as vehicle type, weight, model year, engine type, test cycle, and environmental conditions. The WVU transportable emission testing lab has shown its particular value in measuring in-use emissions of heavy duty vehicles.