Utilisation of non-edible biodiesel in the diesel engine has tremendously increased nowadays. This is due to its renewable nature and produces less pollution than conventional diesel, except for NOx emissions. To overcome the drawbacks, some additional fuel is added to take part in the combustion along with the biodiesel in a dual-fuel mode (DFM). In the present work, an investigation was carried out to assess the effect of gasoline as a manifold injection fuel (MIF) under 0, 10, and 20% energy share (ES) on Prosopis juliflora biodiesel (PJBD) fueled diesel engine in the DFM, and compared with single fuel mode (SFM). The diesel B0 (100% Diesel), and PJBD blends B10 (10% PJBD and 90% diesel), and B20 are used as direct injection fuel (DIF) in three fuel combinations. The experiment was carried out in a common rail direct injection, 3.7 kW stationary single-cylinder diesel engine under a constant speed of 1500 rpm. Compared to diesel SFM, the PJBD SFM shows a slightly lower brake thermal efficiency (BTE), in-cylinder pressure (ICP), and net heat release rate (NHRR) along with lesser emissions of HC, CO, and smoke. Addition of gasoline in DFM results in enhancement of ICP, NHRR, and BTE than normal diesel and biodiesel SFM mode, especially 20% ES of gasoline could give higher results than 10% ES. On the other hand, emissions of HC and CO are considerably increased, and NOx and smoke emissions are significantly reduced in DFM when compared to diesel and biodiesel SFM. The gasoline can significantly alter the combustion characteristics of the diesel engine in DFM. Finally, the addition of gasoline in DFM has positive results in all aspects of performance, combustion, and exhaust emissions, except for CO and HC emissions.