Off-Highway Vehicles (OHVs) — including mining trucks, construction machinery, and agricultural equipment — contribute significantly to greenhouse gas (GHG) emissions and local air pollutants due to their dependence on fossil diesel. Achieving sustainable development goals in off-highway sectors requires transitioning toward alternate fuels that can reduce CO₂, NOₓ, and particulate matter (PM) emissions while maintaining performance and reliability. This paper comprehensively evaluates alternate fuels such as biodiesel, renewable diesel, compressed and liquefied natural gas (CNG/LNG), liquefied petroleum gas (LPG), hydrogen, and alcohol-based blends. Using insights from Service Bulletins, fuel standards, and the Worldwide Fuel Charter, it discusses fuel properties, engine compatibility, operational challenges, sustainability impacts, economic feasibility, safety considerations, and regulatory aspects. Case studies of alternate fuel deployment in OHVs illustrate practical challenges and successes. Recommendations are made for fuel selection, system modifications, and future research to support sustainable operation of OHVs.