This work evaluates a standardized 30-ton, 16 m railbus platform optimized for unelectrified regional service, focusing on propulsion system design and trade-offs between range, cost, and emissions. A MATLAB/Simulink drive-cycle model was developed to simulate energy consumption and component performance under realistic operating conditions. The Erfurt–Rennsteig route in Germany (130 km round trip, gradients up to 6 %) was selected as a representative case study. The model incorporates detailed sub-models for traction motors, lithium-ion batteries (LFP and LTO), fuel storage, fuel cells, and ICE gensets across multiple fuel options (diesel, gasoline, methane, ethanol, methanol, HVO, FAME, and hydrogen). Battery lifetime is estimated using a combined cycle- and calendar-aging model using the rainflow algorithm to extract charge cycles, while cost models include capital, fuel, maintenance, track fees, and staffing. Results show that battery-electric configurations achieve 1 kWh/km energy use, while hybrid systems range from 2–4 kWh/km depending on fuel and secondary power unit. Control strategies that enable deeper cycling of the traction battery reduce fuel consumption by 7–18 %, with further savings possible from larger battery or genset capacities. Well-to-wheel greenhouse gas emissions vary widely: from near-zero for renewable fuels and clean electricity mixes to over 1,000 gCO2/kWh for fossil-based options. Lifecycle cost analysis indicates that while fuel may represent up to 25 % of total costs, track and station fees dominate operational expenses. Autonomous operation could eliminate oboard staffing costs, amounting to 25–35 %.