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Assessing Exhaust Toxicity with Biological Detector: Configuration of Portable Air-Liquid Interface Human Lung Cell Model Exposure System, Sampling Train and Test Conditions

Journal Article
2019-24-0050
ISSN: 2641-9637, e-ISSN: 2641-9645
Published September 09, 2019 by SAE International in United States
Assessing Exhaust Toxicity with Biological Detector: Configuration of Portable Air-Liquid Interface Human Lung Cell Model Exposure System, Sampling Train and Test Conditions
Sector:
Citation: Vojtisek-Lom, M., Pechout, M., Macoun, D., Rameswaran, R. et al., "Assessing Exhaust Toxicity with Biological Detector: Configuration of Portable Air-Liquid Interface Human Lung Cell Model Exposure System, Sampling Train and Test Conditions," SAE Int. J. Adv. & Curr. Prac. in Mobility 2(2):520-534, 2020, https://doi.org/10.4271/2019-24-0050.
Language: English

References

  1. Health Effect Institute 2018 State of Global Air https://www.stateofglobalair.org/sites/default/files/soga-2018-report.pdf
  2. European Environment Agency (EEA) 2018
  3. Nam , K.-M. , Selin , N.E. , Reilly , J.M. , and Paltsev , S. Measuring Loss in Human Welfare Caused by Air Pollution: A CGE Analysis for the European Region Energy Policy 38 9 5059 5071 2010
  4. World Bank and Institute for Health Metrics and Evaluation 2016
  5. Künzli , N. , Kaiser , R. , Medina , S. , Studnicka , M. et al. Public-Health Impact of Outdoor and Traffic-Related Air Pollution: a European Assessment Lancet 356 895 901 2000
  6. Peters , A. , Hampel , R. , Cyrys , J. , Breitner , S. et al. Elevated Particle Number Concentrations Induce Immediate Changes in Heart Rate Variability: A Panel Study in Individuals with Impaired Glucose Metabolism or Diabetes Particle and Fibre Toxicology 12 1 2015
  7. Elder , A. , Gelein , R. , Silva , V. , Feikert , T. et al. Translocation of Inhaled Manganese Oxide Particles to the Central Nervous System Environ. Health Perspect. 114 1172 1178 2006
  8. Oberdörster , G. , Sharp , Z. , Atudorei , V. , Elder , A. et al. Translocation of Inhaled Ultrafine Particles to the Brain Inhal. Toxicol. 16 437 445 2004
  9. IARC - International Agency for Research on Cancer and World Health Organization IARC Monographs on the Evaluation of Carcinogenic Risks to Humans Diesel and Gasoline Engine Exhaust and Some Nitroarenes 2013 http://monographs.iarc.fr/ENG/Monographs/vol105/mono105.pdf
  10. Hesterberg , T.W. et al. Particulate Matter in New Technology Diesel Exhaust (NTDE) Is Quantitatively and Qualitatively Very Different from That Found in Traditional Diesel Exhaust (TDE) Journal of the Air & Waste Management Association 61 9 894 913 2011
  11. Olsen , D.B. , Kohls , M. , and Arney , G. Impact of Oxidation Catalysts on Exhaust NO2/NOx Ratio from Lean-Burn Natural Gas Engines J. Air Waste Manag. Assoc. 60 867 874 2010 10.3155/1047-3289.60.7.867
  12. Carslaw , D.C. Evidence of an Increasing NO2/NOX Emissions Ratio from Road Traffic Emissions Atmos. Environ. 39 2005 10.1016/j.atmosenv.2005.06.023
  13. Faustini , A. , Rapp , R. , and Forastiere , F. Nitrogen Dioxide and Mortality: Review and Meta-Analysis of Long-Term Studies Eur. Respir. J. 44 744 753 2014
  14. US EPA 2015 Integrated Science Assessment for Oxides of Nitrogen - Health Criteria (Second External Review Draft) http://cfpub.epa.gov/ncea/isa/recordisplay.cfm?deid=288043
  15. Brand , C. Beyond ‘Dieselgate’: Implications of Unaccounted and Future Air Pollutant Emissions and Energy Use for Cars in the United Kingdom Energy Policy 97 1 12 2016
  16. Sun , K. et al. Vehicle Emissions as an Important Urban Ammonia Source in the United States and China Environ. Sci. Technol 51 4 2472 2481 2017
  17. Jathar , S.H. , Heppding , C. , Link , M.F. , Farmer , D.K. et al. Investigating Diesel Engines as an Atmospheric Source of Isocyanic Acid in Urban Areas Atmospheric Chemistry and Physics 17 14 8959 8970
  18. Gutzwiller , L. , Arens , F. , Baltensperger , U. , Gäggeler , H.W. et al. Significance of Semivolatile Diesel Exhaust Organics for Secondary HONO Formation Environmental Science & Technology 36 4 677 682 2002 10.1021/es015673b
  19. Kurtenbach , R. et al. Investigations of Emissions and Heterogeneous Formation of HONO in a Road Traffic Tunnel Atmospheric Environment 35 20 3385 3394 2001
  20. Enya , T. et al. 3-Nitrobenzanthrone, a Powerful Bacterial Mutagen and Suspected Human Carcinogen Found in Diesel Exhaust and Airborne Particulates Environmental Science and Technology 31 2772 2776 1997
  21. European Commission 2008
  22. Hinds , W.C. Aerosol Technology: Properties, Behavior, and Measurement of Airborne Particles John Wiley & Sons 2012
  23. Otsuki , Y. , Nakamura , H. , Kojima , K. , Kondo , K. et al. Investigation in Calibration Procedures for Portable and Stationary Solid Particle Number Measurement Systems SAE Technical Paper 2019-01-1187 10.4271/2019-01-1187
  24. Cassee , F.R. , Héroux , M.-E. , Gerlofs-Nijland , M.E. , and Kelly , F.J. Particulate Matter beyond Mass: Recent Health Evidence on the Role of Fractions, Chemical Constituents and Sources of Emissions Inhalation Toxicology. 25 14 802 812 Dec 2013 10.3109/08958378.2013.850127
  25. Biswas , S. , Verma , V. , Schauer , J.J. , Cassee , F.R. et al. Oxidative Potential of Semi-Volatile and Non-volatile Particulate Matter (PM) from Heavy-Duty Vehicles Retrofitted with Emission Control Technologies Environmental Science and Technology 43 10 3905 3912 May 15 2009
  26. Johnson , K.C. , Durbin , T.D. , Jung , H. et al. Evaluation of the European PMP Methodologies during On-Road and Chassis Dynamometer Testing for DPF Equipped Heavy-Duty Diesel Vehicles Aerosol Science and Technology 43 10 962 969 2009 10.1080/02786820903074810
  27. Baldauf , R.W. et al. Ultrafine Particle Metrics and Research Considerations: Review of the 2015 UFP Workshop Int J Environ Res Public Health. 13 11 1054 Nov 2016 10.3390/ijerph13111054
  28. Zhang , Z.-H. and Balasubramanian , R. Physicochemical and Toxicological Characteristics of Particulate Matter Emitted from a Non-road Diesel Engine: Comparative Evaluation of Biodiesel-Diesel and Butanol-Diesel Blends J. Hazard. Mater. 264 395 402 2014
  29. Lawal , A.O. , Zhang , M. , Dittmar , M. , Lulla , A. et al. Heme Oxygenase-1 Protects Endothelial Cells from the Toxicity of Air Pollutant Chemicals Toxicol. Appl. Pharmacol. 284 281 291 2015
  30. Libalova , H. , Rossner , P. , Vrbova , K. , Brzicova , T. et al. Comparative Analysis of Toxic Responses of Organic Extracts from Diesel and Selected Alternative Fuels Engine Emissions in Human Lung BEAS-2B Cells Int. J. Mol. Sci. 17 2016
  31. Bion , A. , Fall , M. , Gouriou , F. , Le Prier , E. et al. Biphasic Culture of Rat Lung Slices for Pharmacotoxicological Evaluation of Complex Atmospheres Cell Biology and Toxicology 18 301 2002
  32. Ghio , A.J. , Dailey , L.A. , Soukup , J.M. , Stonehuerner , J. et al. Growth of Human Bronchial Epithelial Cells at an Air-Liquid Interface Alters the Response to Particle Exposure Part. Fibre Toxicol. 10 25 2013
  33. Gerlofs-Nijland , M.E. , Totlandsdal , A.I. , Tzamkiozis , T. , Leseman , D.L.A.C. et al. Cell Toxicity and Oxidative Potential of Engine Exhaust Particles: Impact of Using Particulate Filter or Biodiesel Fuel Blend Environ. Sci. Technol. 47 5931 5938 2013
  34. Totlandsdal , A.I. , Lag , M. , Lilleaas , E. , Cassee , F. et al. Differential Proinflammatory Responses Induced by Diesel Exhaust Particles with Contrasting PAH and Metal Content Env. Toxicol. 30 188 196 2015
  35. Martin , N. , Lombard , M. , Jensen , K.R. , Kelley , P. et al. Effect of Biodiesel Fuel on “Real-World”, Nonroad Heavy Duty Diesel Engine Particulate Matter Emissions, Composition and Cytotoxicity Science of the Total Environment 586 409 418 2017
  36. Müller , L. , Comte , P. , Czerwinski , J. , Kasper , M. et al. New Exposure System to Evaluate the Toxicity of (Scooter) Exhaust Emissions in Lung Cells In Vitro Environmental and Scientific Technology 44 2632 2638 2010
  37. Steiner , S. , Czerwinski , J. , Comte , P. , Heeb , N.V. et al. Effects of an Iron-Based Fuel-Borne Catalyst and a Diesel Particle Filter on Exhaust Toxicity in Lung Cells In Vitro Anal. Bioanal. Chem. 407 5977 5986 2015
  38. Steiner , S. , Mueller , L. , Popovicheva , O.B. , Raemy , D.O. et al. Cerium Dioxide Nanoparticles Can Interfere with the Associated Cellular Mechanistic Response to Diesel Exhaust Exposure Toxicol. Lett. 214 218 225 2012
  39. Hawley , B. , L’Orange , C. , Olsen , D.B. , Marchese , A.J. , and Volckens , J. Oxidative Stress and Aromatic Hydrocarbon Response of Human Bronchial Epithelial Cells Exposed to Petro- or Biodiesel Exhaust Treated with a Diesel Particulate Filter Toxicol. Sci. 141 505 514 2014
  40. Oeder , S. , Kanashova , T. , Sippula , O. , Sapcariu , S.C. et al. Particulate Matter from both Heavy Fuel Oil and Diesel Fuel Shipping Emissions Show Strong Biological Effects on Human Lung Cells at Realistic and Comparable In Vitro Exposure Conditions PLoS One 10 e0126536 2015
  41. Aufderheide , M. and Mohr , U. CULTEX a New System and Technique for the Cultivation and Exposure of Cells at the Air/Liquid Interface Exp. Toxicol. Pathol. 51 489 490 1999
  42. Savi , M. , Kalberer , M. , Lang , D. , Ryser , M. et al. A Novel Exposure System for the Efficient and Controlled Deposition of Aerosol Particles onto Cell Cultures Environ. Sci. Technol. 42 5667 5674 2008
  43. Anadón , A. , Martínez , M.A. , Castellano , V. , and Martínez-Larrañaga , M.R. The Role of In Vitro Methods as Alternatives to Animals in Toxicity Testing Expert Opin. Drug Metab. Toxicol. 10 67 79 2014
  44. Rothen-Rutishauser , B. , Blank , F. , Mühlfeld , C. , and Gehr , P. In Vitro Models of the Human Epithelial Airway Barrier to Study the Toxic Potential of Particulate Matter Expert Opin. Drug Metab. Toxicol. 4 1075 1089 2008
  45. Steiner , S. , Heeb , N.V. , Czerwinski , J. , Comte , P. et al. Test-Methods on the Test-Bench: A Comparison of Complete Exhaust and Exhaust Particle Extracts for Genotoxicity/Mutagenicity Assessment Environ. Sci. Technol. 48 5237 5244 2014
  46. Lacroix , G. , Koch , W. , Ritter , D. , Gutleb , A.C. et al. Air-Liquid Interface In Vitro Models for Respiratory Toxicology Research: Consensus Workshop and Recommendations Applied In Vitro Toxicology 4 2 91 106 2018
  47. Geiser , M. and Lang , D. In Vitro Replica of the Inner Surface of the Lungs for the Study of Particle-Cell Interactions Altex 24 82 84 2007
  48. Upadhyay , S. and Palmberg , L. Air-Liquid Interface: Relevant in Vitro Models for Investigating Air Pollutant-Induced Pulmonary Toxicity Toxicol. Sci. 164 21 30 2018 10.1093/toxsci/kfy053
  49. Tippe , A. , Heinzmann , U. , and Roth , C. Deposition of Fine and Ultrafine Aerosol Particles during Exposure at the Air/Cell Interface J Aerosol Sci 33 207 218 2002
  50. Bitterle , E. , Karg , E. , Schroeppel , A. et al. Dose-Controlled Exposure of A549 Epithelial Cells at the Air-Liquid Interface to Airborne Ultrafine Carbonaceous Particles Chemosphere 65 1784 1790 2006
  51. Aufderheide , M. , Knebel , J.W. , and Ritter , D. Novel Approaches for Studying Pulmonary Toxicity In Vitro Toxicology Letters 140 205 211 2003
  52. Geiser , M. , Jeannet , N. , Fierz , M. , and Burtscher , H. Evaluating Adverse Effects of Inhaled Nanoparticles by Realistic In Vitro Technology Nanomaterials 7 2 49 2017
  53. Ihalainen , M. , Jalava , P. , Ihantola , T. , Kasurinen , S. et al. Design and Validation of an Air-Liquid Interface (ALI) Exposure Device Based on Thermophoresis Aerosol Science and Technology 1 13 2018
  54. Cooney , D.J. and Hickey , A.J. Cellular Response to the Deposition of Diesel Exhaust Particle Aerosols onto Human Lung Cells Grown at the Air-Liquid Interface by Inertial Impaction Toxicology In Vitro 25 8 1953 1965 2011
  55. Reddel , R.R. , Ke , Y. , Gerwin , B.I. , McMenamin , M.G. et al. Transformation of Human Bronchial Epithelial Cells by Infection with SV40 or Adenovirus-12 SV40 Hybrid Virus, or Transfection Via Strontium Phosphate Coprecipitation with a Plasmid Containing SV40 Early Region Genes Cancer Res 48 1904 1909 1988
  56. Huang , S. , Wiszniewski , L. , Constant , S. , and Roggen , E. Potential of In Vitro Reconstituted 3D Human Airway Epithelia (MucilAirTM) to Assess Respiratory Sensitizers Toxicology In Vitro 27 3 1151 1156 2013
  57. Mazzarella , G. , Ferraraccio , F. , Prati , M.V. , Annunziata , S. et al. Effects of Diesel Exhaust Particles on Human Lung Epithelial Cells: An In Vitro Study Respiratory Medicine 101 6 1155 1162 2007
  58. Kooter , I.M. , Alblas , M.J. , Jedynska , A.D. , Steenhof , M. et al. Alveolar Epithelial Cells (A549) Exposed at the Air-Liquid Interface to Diesel Exhaust: First Study in TNO’s Powertrain Test Center Toxicology In Vitro 27 8 2342 2349 2013
  59. Barraud , C. , Corbière , C. , Pottier , I. , Estace , E. et al. Impact of after-Treatment Devices and Biofuels on Diesel Exhausts Genotoxicity in A549 Cells Exposed at Air-Liquid Interface Toxicology In Vitro 45 426 433 2017
  60. Bisig , C. , Roth , M. , Müller , L. , Comte , P. et al. Hazard Identification of Exhausts from Gasoline-Ethanol Fuel Blends Using a Multi-Cellular Human Lung Model Environmental Research 151 789 796 2016
  61. Douki , T. , Corbière , C. , Preterre , D. , Martin , P.J. et al. Comparative Study of Diesel and Biodiesel Exhausts on Lung Oxidative Stress and Genotoxicity in Rats Environmental Pollution 235 514 524 2018
  62. Okubo , T. , Hosaka , M. , and Nakae , D. In Vitro Effects Induced by Diesel Exhaust at an Air-Liquid Interface in a Human Lung Alveolar Carcinoma Cell Line A549 Experimental and Toxicologic Pathology 67 7-8 383 388 2015
  63. Roth , M. , Usemann , J. , Bisig , C. , Comte , P. et al. Effects of Gasoline and Ethanol-Gasoline Exhaust Exposure on Human Bronchial Epithelial and Natural Killer Cells In Vitro Toxicology in Vitro 45 101 110 2017
  64. Knebel , J.W. , Ritter , D. , and Aufderheide , M. Exposure of Human Lung Cells to Native Diesel Motor Exhaust-Development of an Optimized In Vitro Test Strategy Toxicology In Vitro 16 2 185 192 2002
  65. Pham , L. , Yang , J. , Johnson , K. , Durbin , T. et al. Evaluation of Partial Flow Dilution Systems for Very low PM Mass Measurements Emission Control Science and Technology 4 4 247 259 2018
  66. Homolya , J. and Griffin , R. 1972
  67. Vojtíšek , M. 2009
  68. Ankilov , A. , Baklanov , A. , Colhoun , M. et al. Particle Size Dependent Response of Aerosol Counters Atmospheric Research 62 3-4 209 237 2002
  69. Nowak , A. and Andres , H.P. Measuring Soot Particles from Engines: Recommendations from EMRP-ENV02 Project in WP1 18th ETH Conference on Combustion Generated Nanoparticles ETH Zurich, Switzerland June 22-25 2014 http://www.nanoparticles.ch/2014_ETH-NPC-18/7-5_Nowak.pdf

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