Rapid Engineering of Three-Dimensional, Multicellular Tissues With Polymeric Scaffolds

TBMG-2312

10/01/2007

Abstract
Content

A process has been developed for the rapid tissue engineering of multicellular- tissue-equivalent assemblies by the controlled enzymatic degradation of polymeric beads in a low-fluid- shear bioreactor. In this process, the porous polymeric beads serve as temporary scaffolds to support the assemblies of cells in a tissuelike 3D configuration during the critical initial growth phases of attachment of anchorage-dependent cells, aggregation of the cells, and formation of a 3D extracellular matrix. Once the cells are assembled into a 3D array and enmeshed in a structural supportive 3D extracellular matrix (ECM), the polymeric scaffolds can be degraded in the low-fluid-shear environment of the NASA-designed bioreactor. The natural 3D tissuelike assembly, devoid of any artificial support structure, is maintained in the low-shear bioreactor environment by the newly formed natural cellular/ECM. The elimination of the artificial scaffold allows normal tissue structure and function.

Meta TagsDetails
Citation
"Rapid Engineering of Three-Dimensional, Multicellular Tissues With Polymeric Scaffolds," Mobility Engineering, October 1, 2007.
Additional Details
Publisher
Published
Oct 1, 2007
Product Code
TBMG-2312
Content Type
Magazine Article
Language
English