Neutrophil-Screening Assay Using Two-Color Flow Cytometry

An in vitro test that includes the use of two-color flow cytometry has been developed for use in screening of neutrophils for their ability to fight infections. Neutrophils constitute the first line of defense of the human body against infections. Neutrophils engulf invading bacteria in a process called" phagocytosis." The primary means by which they destroy the bacteria is the production of hydrogen peroxide and toxic oxygen radicals; this production is called "oxidative burst." Acquired defects in phagocytosis or oxidative burst can allow localized or generalized infections to develop. Such defects can be caused by toxins, drugs (including adrenocorticosteroids), and/or radiation. A rapid test that yields data on such defects could be helpful in choosing appropriate therapeutic measures prior to onset of overt clinical disease.