Method and Apparatus for Automated Isolation of Nucleic Acids from Small Cell Samples
TBMG-14875
10/01/2012
- Content
RNA isolation is a ubiquitous need, driven by current emphasis on microarrays and miniaturization. With commercial systems requiring 100,000 to 1,000,000 cells for successful isolation, there is a growing need for a small-footprint, easy-to-use device that can harvest nucleic acids from much smaller cell samples (1,000 to 10,000 cells). The process of extraction of RNA from cell cultures is a complex, multi-step one, and requires timed, asynchronous operations with multiple reagents/buffers. An added complexity is the fragility of RNA (subject to degradation) and its reactivity to surface.
- Citation
- "Method and Apparatus for Automated Isolation of Nucleic Acids from Small Cell Samples," Mobility Engineering, October 1, 2012.