Live Cell Imaging Using Holotomography

Since Robert Hooke's microscopic observations of cork cells in the 17th century, optical imaging has proven an indispensable technique in elucidating the pathophysiology of diseases. Perhaps the real transformation occurred last century with Zernike's invention of the phase-contrast microscope, measuring the variations in light as it passes through transparent specimens to reveal details of the cellular structures without fixing and staining. At a stroke, these detailed 2D images kickstarted the science of cell biology and provided the foundations for the explosive advancements in live cell imaging (LCI) in recent years.