Matrix-Free Assisted Laser Desorption Ionization Using Metal-Organic Frameworks
- Magazine Article
Matrix-assisted laser desorption/ionization (MALDI) is a soft ionization technique that is widely applied in the characterization of large biomolecules using various mass spectrometry (MS) analyzers, specifically, time-of-flight (TOF) analyzers. The MALDI process involves the deposition of an analyte solution onto a metal substrate before the addition of a matrix. The matrix/analyte dry spot is exposed to a UV laser, and the laser energy that is absorbed by the matrix/analyte is converted into heat energy that initiates charge transfer, which results in the desorption of the matrix and analyte molecules in ionized form. The positive ions are then accelerated through a vacuum into MS analyses.