Cylinder block is the bulkiest component in engine. It undergoes severe thermal and mechanical loads. Also, cylinder block is multifunctional as it includes positioning of coolant and lubrication circuit, location of coolant and oil pumps, locating cylinder head to form closed combustion chamber, locating mounting points of engine, mounting of alternator, starting motor, and AC compressors. Hence, the cylinder block design should be the strong and robust enough to handle the forces induced by combustion. Taking this requirement as the base, cylinder block is designed for Horizontal K type configuration. In this research, the mounting points of cylinder block are decided based on the imbalance forces for the configuration and from the gas pressure forces. Rigid body dynamics approach is used for locating the mounting points of engine. Topology optimization is carried out to know the location of coolant circuit. As the engine is subjected to heavy thermo-mechanical loads, initially transient structural analysis is performed on the block to determine the mechanical stress and deformation and subsequently, thermo-mechanical analysis is performed to check for thermal deformation and stresses and also to know the maximum temperature reached. Topology optimization, rigid body dynamics, preload bolt analysis, MODAL analysis, transient structural and thermo-mechanical analysis are performed in ANSYS 2019 R3 software. The positioning of the mounting points are discussed in this paper. Along with this, structural and thermal analysis are performed on the final cylinder block to understand its strength and to know whether the designed block is suitable for the engine working conditions.