In this modern era increase in pollution became a huge impact on the lives of all living creatures, in this automobile tends to be one of the major contributors in terms of air pollution thanks to their exhaust emissions. The objective of the present study is to reduce the amount of harmful pollutants emitted from the automobiles by the utilization of a biofuel further influenced by two additives (liquid and a Nano additive). In this study, first the bio oil is extracted, then the biofuel is mixed with diesel fuel at different proportions of 20%, 40% by volume. Experiments are carried out in a common rail direct injection, diesel engine, which is a stationary test engine manufactured by Kirloskar, connected to a computer setup with an open control unit. The emission values in the exhaust gases are obtained using AVL exhaust gas analyzer. Then 0.1% of rice husk Nano additive addition with the fuel blend followed by 3%, 6% of 1, 4-Dioxane blended with the previous blend and its performance (BTE, BSFC) and emission (HC, CO, CO2, NOx, Smoke) values are tested. The values tabulated and compared for identifying the best blends of them all in terms of emission reduction. Performance of the engine reduced with the addition of the additives for about 4.3% compared with that of diesel fuel. And also, considerable reduction in emission was noticed for HC, CO, Smoke and even for NOx emission with the increase in 1,4-dioxane percentage. The addition of rice husk Nano particles aids in the reduction of emissions. The 40% blend along with 6% and 0.1% of 1,4-dioxane and rice husk shows the lowest possible emission. This comparative analysis helps in understanding the influence of the additives on the biodiesel blends. In addition, the outcome helps in determining the suitability of the combination considered here for use in vehicles without any major modifications.