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Rapid and Accurate Quantification of Bacterial Cells in Fresh Water Using a Simplified Microfluidic Device
Technical Paper
2006-01-2079
ISSN: 0148-7191, e-ISSN: 2688-3627
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English
Abstract
The prevention of waterborne outbreaks associated with contaminated water is one of the most important topics in closed habitation. We investigated the ability of a newly designed microfluidic device to determine bacterial numbers in freshwater. A microfluidic device was designed and fabricated by polydimethylsiloxane (size: 50 mm × 24 mm). A fluorescent microscope-based system was used for enumeration of cells flowing rapidly through the microchannel. The numbers of E. coli cells suspended in filtrated sterilized water at a density in the order of 104 - 106 /ml determined by this on-chip flow cytometer were similar with the microscopic counts. The time required for enumeration by on-chip flow cytometer was approximately 10 min per sample. This simple enumeration method will contribute to the technical progress in microbiological quality control of fresh water used in CELSS.
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Citation
Yamaguchi, N., Nagase, H., and Nasu, M., "Rapid and Accurate Quantification of Bacterial Cells in Fresh Water Using a Simplified Microfluidic Device," SAE Technical Paper 2006-01-2079, 2006, https://doi.org/10.4271/2006-01-2079.Also In
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