This content is not included in your SAE MOBILUS subscription, or you are not logged in.
Rapid and Accurate Quantification of Bacterial Cells in Fresh Water Using a Simplified Microfluidic Device
ISSN: 0148-7191, e-ISSN: 2688-3627
Published July 17, 2006 by SAE International in United States
Annotation ability available
The prevention of waterborne outbreaks associated with contaminated water is one of the most important topics in closed habitation. We investigated the ability of a newly designed microfluidic device to determine bacterial numbers in freshwater. A microfluidic device was designed and fabricated by polydimethylsiloxane (size: 50 mm × 24 mm). A fluorescent microscope-based system was used for enumeration of cells flowing rapidly through the microchannel. The numbers of E. coli cells suspended in filtrated sterilized water at a density in the order of 104 - 106 /ml determined by this on-chip flow cytometer were similar with the microscopic counts. The time required for enumeration by on-chip flow cytometer was approximately 10 min per sample. This simple enumeration method will contribute to the technical progress in microbiological quality control of fresh water used in CELSS.
CitationYamaguchi, N., Nagase, H., and Nasu, M., "Rapid and Accurate Quantification of Bacterial Cells in Fresh Water Using a Simplified Microfluidic Device," SAE Technical Paper 2006-01-2079, 2006, https://doi.org/10.4271/2006-01-2079.
- McFeters G. A. “Enumeration, occurrence, and significance of injured indicator bacteria in drinking water” Drinking water microbiology: progress and recent developments McFeters G. A. New York Springer-Verlag 1990 478 492
- Kirchman D. Sigda J. Kapuscinski R. Mitchell R. “Statistical analysis of the direct count method for enumerating bacteria” Appl. Environ. Microbiol. 44 : 376 382 1982
- Vives-Rego J. Lebaron P. Caron G. N. “Current and future application of flow cytometry in aquatic microbiology” FEMS Microbiol. Rev. 24 : 429 448 2000
- Blankenstein G. Larsen U. D. “Modular concept of a laboratory on a chip for chemical and biochemical analysis” Biosens. Bioelectron. 13 : 427 438 1988
- Sakamoto C. Yamaguchi N. Nasu M. “Rapid and simple quantification of bacterial cells by using a microfluidic device” Appl. Environ. Microbiol. 71 : 1117 1121 2005
- Hobbie J. E. Daley R. J. Jasper S. “Use of nucleopore filters for counting bacteria by fluorescence microscopy” Appl. Environ. Microbiol. 33 : 1225 1228 1977