This content is not included in
your SAE MOBILUS subscription, or you are not logged in.
Passive Observatories for Experimental Microbial Systems (POEMS): Microbes Return to Flight
Technical Paper
2005-01-2984
ISSN: 0148-7191, e-ISSN: 2688-3627
Annotation ability available
Sector:
Language:
English
Abstract
For long-duration exploration missions where re-supply is not a viable alternative for human crews, sustainable strategies based on integrated bioprocesses and physical-chemical systems are required. Bioregenerative life support elements enabling human exploration systems require microbial communities that are physiologically diverse, functionally stable, and non virulent. Given the potential for rapid change in microbial populations through processes that may be accelerated in space (i.e., mutation, recombination, and natural selection), multi-generation experiments are required to understand the pattern and process of microbial community assembly and evolution in the space environment. In order to advance the technology readiness level of biological systems for exploration missions, these studies should enable independent examination of gravity and radiation effects in the space environment at multiple levels of organization from individual cell growth to ecosystem ecology. One requirement for these studies is a cell cultivation unit with low mass, volume, energy, and crew-time requirements for the multi-generation growth of microbial populations in the space environment. Passive Observatories for Experimental Microbial Systems (POEMS) are an integrated flight-ready unit to support multi-generation microbial studies. The POEMS cultivation unit integrates the OptiCell™, a small sterile growth chamber sealed between optically clear gas-permeable growth surfaces, with modified BRIC (Biological Research in a Canister) flight hardware. Each BRIC-Opti canister provides mechanical support for four replicate OptiCell™ chambers, a captured volume of atmosphere for microbial metabolism, gas sampling capability, and autonomous temperature data logging during the mission. The POEMS experiment will enable analyses of microbial growth and evolution in a microgravity environment on STS-121 during ULF1.1 and on ISS during Increment 11.
Recommended Content
Authors
Topic
Citation
Roberts, M., Reed, D., and Rodriguez, J., "Passive Observatories for Experimental Microbial Systems (POEMS): Microbes Return to Flight," SAE Technical Paper 2005-01-2984, 2005, https://doi.org/10.4271/2005-01-2984.Also In
References
- Baker, P. Leff L. 2005 Intraspecific differences in bacterial responses to modelled reduced gravity J. Appl. Microbiol. 98 1239 1246
- Baker, P. Leff L. 2004 The effect of simulated microgravity on bacteria from the Mir Space Station Microgravity Sci. Technol. 15 35 41
- Blackwood, K. S. Turenne C. Y. Harmsen D. Kabani A. M. 2004 Reassessment of sequence-based targets for identification of Bacillus species J. Clin. Micro. 42 1626 1630
- Borchers, A. T. Keen C. L. Gershwin M. E. 2002 Microgravity and immune responsiveness: implications for space travel Nutrition. 18 889 98
- Brenner, D. J. Elliston C. D. 2001 The potential impact of bystander effects on radiation risks in a mars mission Radiat. Res. 156 612 617
- Canova, S. Fiorasi F. Mognato M. Grifalconi M. Reddi E. Russo A. Celottia L. 2005 “Modeled Microgravity” Affects Cell Response to Ionizing Radiation and Increases Genomic Damage. Radiation Research Radiation Research 163 191 199
- Castro, V. A. Thrasher A. N. Healy M. Ott C. M. Pierson D. L. 2004 Microbial characterization during the early habitation of the International Space Station Microb. Ecol. 47 119 26
- Cioletti, L. A. Pierson D. L. Mishra S. K. 1991 Microbial growth and physiology in space: A review 21st International Conference of Environmental Systems (ICES) SAE 911512
- Criswell-Hudak, B. S. 1991 Immune response during space flight Exp. Gerontol. 26 289 296
- Curtis, S. B. Letaw J. W. 1989 Galactic cosmic rays and cell-hit frequencies outside the magnetosphere Adv. Space Res. 9 293 298
- Demain, A. L. Fang A. 2001 Secondary Metabolism in Simulated Microgravity The Chemical Record 1 333 346
- Kacena, M. A. Todd P. 1997 Growth characteristics of Escherichia coli and Bacillus subtilis cultured on an agar substrate in microgravity Microgravity Sci. Technol. 10 58 62
- Kacena, M. A. Leonard P. E. Todd P. Luttges M. W. 1997 Low gravity and inertial effects on the growth of Escherichia coli and Bacillus subtilis in semi-solid media Aviat. Space. Environ. Med. 68 1104 1108
- Kacena, M. A. Manfredi B. Todd P. P. 1999a Effects of space flight and mixing on bacterial growth in low volume cultures Microgravity Sci. Technol. 12 74 77
- Kacena, M. A. Merrell G. A. Manfredi B. Smith E. E. Klaus D. M. Todd P. 1999b Bacterial growth in space flight: logistic growth curve parameters for Escherichia coli and Bacillus subtilis Appl. Environ. Microbiol. 51 229 234
- Klaus, D. M. Luttges M. W. Stodieck L. S. 1994 Investigation of space flight effects on Escherichia coli growth 24th International Conference of Environmental Systems (ICES) SAE 941260
- Lam, K. S. Gustavson D. R. Pirnik D. L. Pack E. Bulanhagui C. Mamber S. W. Forenza S. Stodieck L. S. Klaus D. M. 2002 The effect of space flight on the production of actinomycin D by Streptomyces plicatus J. Ind Microbiol and Biotech. 29 299 302
- Lam, K. S. Mamber S. Pack E. Forenza S. Fernandes P. Klaus D. M. 1998 The effects of space flight on the production of monorden by Humicola fuscoatra WC5157 in solid-state fermentation Appl. Microbiol. and Biotech. 49 579 583
- Lynch, S. V. Brodie E. L. Matin A. 2004 Role and regulation of σ s in general resistance conferred by Low-Shear Simulated Microgravity in Escherich ia coli J. Bacteriol. 186 8207 8212
- McPhee, J. C. White R. J. 2003 Physiology, medicine, long-duration space flight and the NSBRI Acta Astronaut. 53 239 248
- National Research Council 1996 Radiation Hazards to Crews on Interplanetary Missions: Biological Issues and Research Strategies National Academy Press Washington, DC, USA
- Nickerson, C. A. Ott C. M. Wilson J. W. Ramamurthy R. Pierson D. L. 2004 Microbial responses to microgravity and other low-shear environments Microbiol. Mol. Biol. Rev. 68 345 361
- Nickerson, C. A. Ott C. M. Wilson J. W. Ramamurthy R. LeBlanc C. L. Honer zu Bentrup K. Hammond T. Pierson D. L. 2003 Low-shear modeled microgravity: a global environmental regulatory signal affecting bacterial gene expression, physiology, and pathogenesis J. Microbiol. Methods. 54 1 11
- Roberts, M. S. Garland, J.L. 2003 Passive Experimental Microbial Systems: A research platform for the analysis of microbial community assembly in spaceflight ecosystems 33rd International Conference of Environmental Systems (ICES) SAE 2003-01-2510
- Roberts, M. S. Garland J. L. Mills A. L. 2004 Microbial Astronauts: Assembling microbial communities for advanced life support systems Microb. Ecol. 47 137 149
- Setlow, R. B. 2003 The hazards of space travel EMBO reports 4 1013 1016
- Smith, S. M. Zwart S. R. Block G. Rice B. L. Davis-Street J. E. 2005 The nutritional status of astronauts is altered after long-term space flight aboard the International Space Station J. Nutrition. 135 437 443
- Taylor, G. R. Dardano J. R. 1984 Human cellular immune responsiveness following space flight Kosm. Biol. Aviakosm. Med. 18 74 80
- Wilson, J. W. Ott C. M. Ramamurthy R. Porwollik S. McClelland M. Pierson D. L. Nickerson C. A. 2002a Low-shear modeled microgravity alters the Salmonella enterica serovar Typhimuriu m stress response in an RpoS-independent manner Appl. Environ. Microbiol. 68 5408 5416
- Wilson, J. W. Ramamurthy R. Porwollik S. McClelland M. Hammond T. Allen P. Ott C. M. Pierson D. L. Nickerson C. A. 2002b Microarray analysis identifies Salmonella genes belonging to the low-shear modeled microgravity regulon Proc. Natl. Acad. Sci. USA 99 13807 13812