This content is not included in your SAE MOBILUS subscription, or you are not logged in.
Transgenic Plant Biomonitors: Stress Gene Biocompatibility Evaluation of the Plant Growth Facility for PGIM-01
ISSN: 0148-7191, e-ISSN: 2688-3627
Published July 09, 2001 by SAE International in United States
Annotation ability available
The initial task in the preparation for Flight Experiment PGIM-01 (Plant Growth in Microgravity - 01) was the optimization of the plant nutrient system within the environment of the Plant Growth Facility (PGF – a Space Shuttle middeck locker plant growth unit). PGIM-01 entailed using the Transgenic Arabidopsis Gene Expression System (TAGES) to monitor effects of spaceflight-associated stress on gene expression. TAGES plants are genetically engineered arabidopsis plants designed with a sensitive reporter gene system that responds to a variety of environmental stresses. However, transgene expression can also be influenced by background environmental conditions. Thus, minimizing sources of background stress on the plants was crucial to ensure optimal growth and a high scientific return.
CitationPaul, A., Daugherty, C., Bihn, E., Ferl, R. et al., "Transgenic Plant Biomonitors: Stress Gene Biocompatibility Evaluation of the Plant Growth Facility for PGIM-01," SAE Technical Paper 2001-01-2181, 2001, https://doi.org/10.4271/2001-01-2181.
- Chapman, D. K. Wells, H. W. 1996 Development of the plant growth facility for the use in the shuttle middeck and test units for ground-based experiments In Proc. Sixth European Symp. on Life Sci. Res. in Space Trondheim Norway 37 41
- Chapman, D. K. Wells, H. W. Levine, H. G. 1995
- Chung, H. J. Ferl, R. J. 1999 Arabidopsis alcohol dehydrogenase expression in both shoots and roots is conditioned by root growth environment Plant Physiol 121 429 436
- Dolferus, R. Jacobs, M. Peacock, W. J. Dennis, E. S. 1994 Differential interactions of promoter elements in stress responses of the Arabidopsis Adh gene Plant Physiol 105 1075 1087
- Ellis, M. H. Dennis, E. S. Peacock, W. J. 1999 Arabidopsis roots and shoots have different mechanisms for hypoxic stress tolerance Plant Physiol 119 57 64
- Murashige, T. Skoog, F. 1962 A revised medium for rapid growth and bioassays with tobacco tissue culture Physiol. Plant. 15 473 479
- Paul, A.-L. Semer, C. Kucharek, T. Ferl, R. J. 2001a The fungicidal and phytotoxic properties of benomyl and PPM in supplemented agar media supporting transgenic arabidopsis plants for a Space Shuttle flight experiment Appl. Microbiol. and Biotechnol. 55 480 485
- Paul, A.-L. Daughtery, C. J. Bihn, E. A. Chapman, D. K. Norwood, K. L. L. Ferl, R. J. 2001b June 2001