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Phylogenetic Characterization and Description of Novel-tolerant Bacillus Species Isolated from Spacecraft Assembly Facility
Technical Paper
2000-01-2419
ISSN: 0148-7191, e-ISSN: 2688-3627
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English
Abstract
Microbial biofilms representing both Gram-positive and Gram-negative bacteria were artificially coated onto aluminum metal surfaces that are commonly used in building spacecraft. Changes in bacterial numbers were monitored over time on metal test sections by conventional spread plate technique and polymerase chain reaction (PCR). Conventional standard plating technique did not recover desiccated bacterial biomass from either glass or aluminum metal surfaces even with a density of 106 cells per coupon. However, whole-cell PCR (without extracting DNA) showed a positive signal for a density of 105 dehydrated microbial cells. Electron and epifluorescent microscopic examinations revealed that bacteria entered a non-cultivable state once the water activity was reduced to minimum. However, a simple DNA extraction protocol combined with PCR amplification of a specific 16S rDNA fragment improved the recovery of non-viable desiccated microbial biofilm from the metal test sections. In ongoing investigations to map and archive the microbial footprints in various components of spacecraft and its accessories, we examined the microbial populations of the JPL-Spacecraft Assembly Facility (SAF). We exposed witness plates that are made up of spacecraft materials and/or painted with spacecraft-quality paints for ~7 to 9 months. In the initial studies reported here, we examined the total cultivable aerobic heterotrophs. The results showed that the witness plates coated with spacecraft quality paints attracted more dust particles than the non-coated stainless steel witness plates. Among four paints tested, witness plates coated with NS43G (an off-white conductive paint [silicate binder]) accumulated the highest number of particles, hence attracting more cultivable microbes and spore-formers. The microbiological examination revealed that the SAF High Bay-1 harbors mainly Gram-positive microbes, the majority being spore-forming Bacillus species. The phylogenetic relationships among these heat-tolerant microbes were examined using a battery of morphological, physiological, molecular and chemotaxonomic characterizations. Using phenotypic characterization alone, only 9 strains were identified. By 16S rDNA analysis, the isolates fell into seven clades: Bacillus licheniformis, B. pumilus, B. cereus, B. circulans, Staphylococcus capitis, Planococcus sp. and Micrococcus lylae.
Authors
- K. Venkateswaran - Planetary Protection Technologies, Jet Propulsion Lab.,California Institute of Technology
- C. Echeverria - Planetary Protection Technologies, Jet Propulsion Lab.,California Institute of Technology
- A. Vu - Planetary Protection Technologies, Jet Propulsion Lab.,California Institute of Technology
- M. Musick - Planetary Protection Technologies, Jet Propulsion Lab.,California Institute of Technology
- S. Chung - Planetary Protection Technologies, Jet Propulsion Lab.,California Institute of Technology
- R. Koukol - Planetary Protection Technologies, Jet Propulsion Lab.,California Institute of Technology
- R. Kern - Planetary Protection Technologies, Jet Propulsion Lab.,California Institute of Technology
- D. C. White - Planetary Protection Technologies, Jet Propulsion Lab.,California Institute of Technology
- M. Satomi
Topic
Citation
Venkateswaran, K., Echeverria, C., Vu, A., Musick, M. et al., "Phylogenetic Characterization and Description of Novel-tolerant Bacillus Species Isolated from Spacecraft Assembly Facility," SAE Technical Paper 2000-01-2419, 2000, https://doi.org/10.4271/2000-01-2419.Also In
References
- Achouak, W. Normand P. Heulin T. 1999 Comparative phylogeny of rrs and nifH genes in the Bacillaceae Int J Syst Bacteriol 49 961 967
- 1980 NASA Standard Procedures for the Microbiological Examination of Space Hardware NHB 5340.1B, 1980, Jet Propulsion Laboratory communication, National Aeronautical and Space Administration
- Ash, C. Priest F. G. Collins M. D. 1993-94 Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Proposal for the creation of a new genus Paenibacillus. Antonie Van Leeuwenhoek 64 253 260
- Claus, D. Berkeley R. C. W. 1986 Genus Bacillus Cohan, 1982 In Bergey's Manual of Sytematic Bacteriology 1105 1139 Sneath P. H. A. 2 Baltimore, MA The Williams & Wilkins Co.
- Cole R. M. Popkin T. J. 1981 Electron microscopy. In Manual of Methods for General Bacteriology 34 51 Gerhardt P. Murray, R.G.E. Costilaw, R.N. Nester, E.W. Wood, W.A. Krieg N.R. Phillips G.B. American Society for Microbiology Washington, D.C
- Johnson J. L. 1981 Genetic characterization. In Manual of Methods for General Bacteriology 450 472 Gerhardt P. Murray, R.G.E. Costilaw, R.N. Nester, E.W. Wood, W.A. Krieg N.R. Phillips G.B. ASM Washington, D.C. American Society for Microbiology
- Kepner R. L. Jr Pratt J. R. et al. 1994 Use of fluorochromes for direct enumeration of total bacteria in environmental samples: past and present Microbiol Rev 58 603 615
- Priest F. G. 1993 Systematics and Ecology of Bacillus Bacillus subtilis and other Gram-positive bacteria 3 33 Sonenshein A. L. Hoch J. A. Losick R. ASM Washington D.C. American Society for Microbiology.
- Puleo, J. R. Favero, M. S. Oxborrow G. S. Herring C. M. 1975 Method for collecting naturally occurring airborne bacterial spores for determining their thermal resistance Appl Microbiol 30 786 790
- Ringelberg, D. B. Townsend, G. T. DeWeerd, K. A. Suflita J. M. White D.C. 1994 Detection of the anaerobic dechlorinating microorganism Desulfomonile tiedjei in environmental matrices by its signature lipopolysaccharide branched-long-chain hydroxy fatty acids FEMS Microbiol Ecol 14 9 18
- Ruimy, R. Breittmayer, V. ElBaze, P. Lafay, B. Boussemart, O. Gauthier M. Christen R. 1994 Phylogenic analysis and assessment of the genera Vibrio, Photobacterium, Aeromonas, and Plesiomonas deduced from small subunit rRNA sequences Int J Syst Bacteriol 44 416 426
- Sambrook, J. Fritsch E. F. Maniatis T. 1989 Molecular Clonig: a Laboratory Manual 2nd edn. Cold Spring Harbor, NY Cold Spring Harbor Laboratory
- Satomi, M. Kimura, B. Mizoi, M. Sato T. Fujii T. 1997 Tetragenococcus muriaticus sp. nov., a new moderately halophilic lactic acid bacterium isolated from fermented squid liver sauce Int J Syst Bacteriol 47 832 836
- Swofford D. 1990 PAUP: phylogenetic analysis using parsimony Computer program distributed by the Illinois Natural History Survey Champaign
- Venkateswaran, K. Nakano, H. Okabe, T. Takayama, K. Matsuda O. Hashimoto H. 1989 Occurrence and distribution of Vibrio spp., Listonella spp., and Clostridium botulinum in the Seto Inland Sea of Japan Appl Environ Microbiol 55 559 567
- Venkateswaran, K. Dollholph, M. E. Aller, R. Stackebrandt, E. Nealson K. 1998 Shewanella amazonensis (sp., nov.), a novel metal-reducing facultative anaerobe from Amazon shelf muds Intern. J. Syst. Bacteriol. 48 965 972