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Implications of the VBNC State of B. cepacia and S. maltophilia on Bioreduction and Microbial Monitoring of ISS Potable Waters
Technical Paper
2005-01-2933
ISSN: 0148-7191, e-ISSN: 2688-3627
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English
Abstract
Certain Eubacteria enter a viable but nonculturable (VBNC) state upon encountering unfavorable environmental conditions. VBNC cells do not divide on conventional media yet remain viable and in some cases retain virulence. Here, we describe the VBNC state of two opportunistic pathogens previously isolated from ISS potable waters, Burkholderia cepacia and Stenotrophomonas maltophilia. Artificially inoculated microcosms were exposed to the biocidal agents copper (CuSO4) and iodine (I2) in an attempt to induce nonculturablility. Viability was assessed via fluorescent microscopy (direct viable count assay coupled with BacLight™ staining) and metabolic activity was monitored by quantifying both intracellular ATP and transcribed rRNA (reverse transcriptase quantitative PCR). Culturablility was lost in both B. cepacia and S. maltophilia within two days of exposure to copper or high concentrations of iodine (6 or 8 ppm). Although both biocides appear effective in eliminating S. maltophilia populations, viable cells were still observed in B. cepacia microcosms through the end of the experiment. The ability of B. cepacia and other microbes to enter a VBNC state and circumvent detection by conventional cultivation-based techniques has serious repercussions. Accurate assessments of the microbial burden in these drinking water systems cannot be made when relying solely on conventional approaches. This study exemplifies the importance of developing and implementing cultivation-independent microbial monitoring techniques.
Authors
- Tara Stuecker - Jet Propulsion Laboratory, California Institute of Technology
- David Newcombe - Jet Propulsion Laboratory, California Institute of Technology
- Myron T. La Duc - Jet Propulsion Laboratory, California Institute of Technology
- Eva Murdock - Jet Propulsion Laboratory, California Institute of Technology
- Randall Sumner
- Kasthuri Venkateswaran
Citation
Stuecker, T., Newcombe, D., La Duc, M., Murdock, E. et al., "Implications of the VBNC State of B. cepacia and S. maltophilia on Bioreduction and Microbial Monitoring of ISS Potable Waters," SAE Technical Paper 2005-01-2933, 2005, https://doi.org/10.4271/2005-01-2933.Also In
References
- Alexander, E. Pham D. Steck T. R. 1999 The viable-but-nonculturable condition is induced by copper in Agrobacterium tumefaciens and Rhizobium leguminosarum Appl. Environ. Microbiol. 65 3754 6
- Amann, R. I. Ludwig W. Schleifer K. H. 1995 Phylogenetic identification and in situ detection of individual microbial cells without cultivation Microbiol. Rev. 59 143 69
- Anderson, R. L. Vess R. W. Panlilio A. L. Favero M. S. 1990 Prolonged survival of Pseudomonas cepacia in commercially manufactured povidone-iodine Appl. Environ. Microbiol. 56 3598 600
- Anonymous 1980 NASA standard procedure for the microbiological examination of space hardware, NHB 5340.1B National Aeronautics and Space Administration
- Byrd, J. J. Xu H. S. Colwell R. R. 1991 Viable but nonculturable bacteria in drinking water Appl. Environ. Microbiol. 57 875 8
- Castro, V. A. Thrasher A. N. Healy M. Ott C. M. Pierson D. L. 2004 Microbial characterization during the early habitation of the International Space Station Microb. Ecol. 47 119 26
- Cohen, J. E. Tilman D. 1996 Biosphere 2 and biodiversity: the lessons so far Science 274 1150 1
- Colwell, R. R. Brayton P. R. Grimes D. J. Roszak D. B. Huq A. Palmer L. M. 1985 Viable but non-culturable Vibrio cholerae and related pathogens in the environment: implications for the release of genetically engineered microorganisms Biotechnology 3 817 820
- Colwell, R. R. Brayton P. R. Herrington D. Tall B. Huq A. Levine M. M. 1996 Viable but nonculturable Vibrio cholerae 01 revert to a culturable state in the human intestine World J. Microbiol. Biotechnol. 12 28 31
- Colwell, R. R. Grimes D. J. 2000 Nonculturable microorganisms in the environment ASM Press Washington, D.C.
- Donlan, R. M. Costerton J. W. 2002 Biofilms: survival mechanisms of clinically relevant microorganisms Clin. Microbiol. Rev. 15 167 93
- Grey, B. Steck T. R. 2001 Concentrations of copper thought to be toxic to Escherichia coli can induce the viable but nonculturable condition Appl. Environ. Microbiol. 67 5325 7
- Huang, C. H. Jang T. N. Liu C. Y. Fung C. P. Yu K. W. Wong W. W. 2001 Characteristics of patients with Burkholderia cepacia bacteremia J. Microbiol. Immunol. Infect. 34 215 9
- Kawamura, Y. Li Y. Liu H. Huang X. Li Z. Ezaki T. 2001 Bacterial population in Russian space station “Mir” Microbiol. Immunol. 45 819 28
- Kell, D. B. Kaprelyants A. S. Weichart D. H. Harwood C. R. Barer M. R. 1998 Viability and activity in readily culturable bacteria: a review and discussion of the practical issues Antonie Van Leeuwenhoek 73 169 87
- Koenig, D. W. Mishra S. K. Pierson D. L. 1995 Removal of Burkholderia cepacia biofilms with oxidants Biofouling 9 51 62
- Koenig, D. W. Pierson D. L. 1997 Microbiology of the Space Shuttle water system Water Sci. Technol. 35 59 64
- Kogure, K. Simidu U. Taga N. 1979 A tentative direct microscopic method for counting living marine bacteria Can. J. Microbiol. 25 415 20
- La Duc, M. T. Sumner R. Pierson D. L. Venkat P. Venkateswaran K. 2004 Evidence of pathogenic microbes in the International Space Station drinking water: reason for concern? Habitation 10 39 48
- Lai, C. H. Chi C. Y. Chen H. P. Chen T. L. Lai C. J. Fung C. P. Yu K. W. Wong W. W. Liu C. Y. 2004 Clinical characteristics and prognostic factors of patients with Stenotrophomonas maltophilia bacteremia J. Microbiol. Immunol. Infect. 37 350 8
- Lleo, M. M. Pierobon S. Tafi M. C. Signoretto C. Canepari P. 2000 mRNA detection by reverse transcription-PCR for monitoring viability over time in an Enterococcus faecalis viable but nonculturable population maintained in a laboratory microcosm Appl. Environ. Microbiol. 66 4564 7
- Madigan, M. T. Martinko J. M. Parker J. 1997 Brock Biology of Microorganisms 8 Prentice Hall Upper Saddle River, NJ
- McKenney, D. Allison D. G. 1995 Effects of growth rate and nutrient limitation on virulence factor production in Burkholderia cepacia J. Bacteriol. 177 4140 3
- Nystrom, T. 2001 Not quite dead enough: on bacterial life, culturability, senescence, and death Arch. Microbiol. 176 159 64
- Pierson, D. L. 2001 Microbial contamination of spacecraft Gravitat. Space Biol. Bull. 14 1 6
- Pommepuy, M. Butin M. Derrien A. Gourmelon M. Colwell R. R. Cormier M. 1996 Retention of enteropathogenicity by viable but nonculturable Escherichia coli exposed to seawater and sunlight Appl. Environ. Microbiol. 62 4621 6
- Rahman, I. Shahamat M. Chowdhury M. A. Colwell R. R. 1996 Potential virulence of viable but nonculturable Shigella dysenteriae type 1 Appl. Environ. Microbiol. 62 115 20
- Suzuki, M. T. Taylor L. T. DeLong E. F. 2000 Quantitative analysis of small-subunit rRNA genes in mixed microbial populations via 5′-nuclease assays Appl. Environ. Microbiol. 66 4605 14
- Taylor, G. Graves R. Brockett R. Ferguson J. Meiszkuc B. 1977 Skylab environmental and crew microbiological studies National Aeronautics and Space Administration
- Venkateswaran, K. Hattori N. La Duc M. T. Kern R. 2003 ATP as a biomarker of viable microorganisms in clean-room facilities J. Microbiol. Methods 52 367 77