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Single Loop for Cell Culture (SLCC) – Development and Spaceflight Qualification of a Perfusion Cell Culture System
Technical Paper
2006-01-2212
ISSN: 0148-7191, e-ISSN: 2688-3627
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English
Abstract
Single Loop for Cell Culture (SLCC) consists of individual, self-contained, spaceflight cell culture systems with capabilities for automated growth initiation, feeding, sub-culturing and sampling. The cells are grown and contained within a rigid cell specimen chamber (CSC). Bladder tanks provide flush and media fluid. SLCC uses active perfusion flow to provide nutrients and gas exchange, and to dilute waste products by expelling depleted media fluid into a waste bladder tank. The cells can be grown quiescently, or suspended using magnetically coupled stirrers. This paper describes the functional and safety design features, the operational modes and the spaceflight qualification processes including science validation tests, using yeast as a model organism.
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Authors
- Alexander Hoehn - BioServe Space Technologies, Aerospace Engineering Sciences, University of Colorado
- Jacob Freeman - BioServe Space Technologies, Aerospace Engineering Sciences, University of Colorado
- Paul Koenig - BioServe Space Technologies, Aerospace Engineering Sciences, University of Colorado
- Louis Stodieck - BioServe Space Technologies, Aerospace Engineering Sciences, University of Colorado
- Matthew Vellone - BioServe Space Technologies, Aerospace Engineering Sciences, University of Colorado
- Shea Williams - BioServe Space Technologies, Aerospace Engineering Sciences, University of Colorado
- Wendy Feenstra - Payload Systems Inc
- Javier de Luis - Payload Systems Inc
- Joe C. Parrish - Payload Systems Inc
- Stephan Pretorius - Payload Systems Inc
- Robert Renshaw - Payload Systems Inc
- Liping Sun - Payload Systems Inc
- Stephen Dyble - NASA Ames Research Center
- Nancy D. Searby - NASA Ames Research Center
- Donald Vandendriesche - NASA Ames Research Center
Topic
Citation
Hoehn, A., Freeman, J., Koenig, P., Stodieck, L. et al., "Single Loop for Cell Culture (SLCC) – Development and Spaceflight Qualification of a Perfusion Cell Culture System," SAE Technical Paper 2006-01-2212, 2006, https://doi.org/10.4271/2006-01-2212.Also In
References
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