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Rapid and Accurate Determination of Bacterial Abundance and their Physiological Activity in Freshwater used in Closed Ecology Experiment Facilities (CEEF) “Mini-Earth”, Japan
Technical Paper
2006-01-2078
ISSN: 0148-7191, e-ISSN: 2688-3627
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English
Abstract
In space utilization, an enormous amount of freshwater for drinking, daily use, hydroponics. is used and recycled in a closed habitat. We have developed culture-independent techniques to analyze microbial cells at the single level, a cell itself, and also community level. By using these methods such as fluorescent vital staining (double staining with carboxyfluorescein diacetate and 4′,6-diamidino-2-phenyl indole), microcolony method and denaturing gradient gel electrophoresis (DGGE), bacterial abundance and their physiological activity in freshwater used in Closed Ecology Experiment Facilities (CEEF) “Mini-Earth” were determined.
Authors
Citation
Baba, T., Yamaguchi, N., Nasu, M., Aibe, Y. et al., "Rapid and Accurate Determination of Bacterial Abundance and their Physiological Activity in Freshwater used in Closed Ecology Experiment Facilities (CEEF) “Mini-Earth”, Japan," SAE Technical Paper 2006-01-2078, 2006, https://doi.org/10.4271/2006-01-2078.Also In
References
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