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Evaluation of Sample Preservation Methods for Space Missions
Technical Paper
2003-01-2671
ISSN: 0148-7191, e-ISSN: 2688-3627
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English
Abstract
This study of samples collected from Mars 01 Orbiter was conducted to gain a better understanding and practical experience in methods to process and preserve samples intended for planetary protection analysis. Samples were evaluated for the viable growth of microbes, the molecular biomarker adenosine triphosphate (ATP), and the presence of lipopolysaccharide, a bacterial cell wall component. Losses were observed in the number of viable microbes after freezing as well as in the detectable lipopolysaccharide. Two independent studies of pooled cleanroom samples demonstrate good ATP recovery and consistent values after freezing at −20 °C.
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Citation
Schubert, W., Kazarians, G., and Rohatgi, N., "Evaluation of Sample Preservation Methods for Space Missions," SAE Technical Paper 2003-01-2671, 2003, https://doi.org/10.4271/2003-01-2671.Also In
References
- Archiving of Biological Samples Collected from Mars 01 Orbiter and KSC Facility Schubert Wayne Rohatgi Naresh Sangari Caroline Quigley Megan LaDuc Myron Chen Fei Venkateswaran Kasthuri Koukol Robert JPL D-22010 2001
- NPG: 5340.1C NASA Standard Procedures for the Microbial Examination of Space Hardware
- Bond W. W. Favero M. S. Korber M. R. 1973 Bacillus sp. ATCC 27380: a spore with extreme resistance to dry heat Applied Microbiology 26 614 616