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Bacterial and Fungal Communities in BPS Chambers and Root Modules
ISSN: 0148-7191, e-ISSN: 2688-3627
Published July 07, 2003 by SAE International in United States
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The PESTO (Photosynthetic Experiment System Testing and Operation) experiment flew in the Biomass Production System (BPS) to International Space Station (ISS) on STS-110 (Atlantis) April 8, 2002, and returned on STS-111 (Endeavour) June 19, 2002, after 73 days in space. The ground control was conducted on a two-week delay at Kennedy Space Center in a BPS unit under environmental conditions comparable to ISS. Wheat (Triticum aestivum cv Apogee) and Brassica rapa cv Astroplant were independently grown in root modules for multiple grow-outs. On-orbit harvests, root modules exchanges and primings, seeds imbibitions, and gas and water samplings occurred at periodic intervals; all were replicated in ground controls. Many operations required crew handling and open access to individual chambers, allowing the exchange of microorganisms between the crew environment and the BPS modules. Upon landing, BPS surfaces, containment bags, root modules, and plant material were swabbed for recovery of microbes. Water samples were collected from BPS nutrient delivery and humidity control systems (HCS), root modules, and wastewater bags. Swabs and liquid samples were plated onto selective media; microbial and fungal colonies were identified to the species level by metabolic profiling when possible. Differences in bacterial and fungal species and the number of species identified were detected between the flight and ground controls.
CitationFrazier, C., Simpson, J., Roberts, M., Stutte, G. et al., "Bacterial and Fungal Communities in BPS Chambers and Root Modules," SAE Technical Paper 2003-01-2528, 2003, https://doi.org/10.4271/2003-01-2528.
- Ferguson James K. Taylor Gerald R. Mieszkuc Bernard J. 1975 Microbiological Results of Apollo, Chapter 2, Microbiological Investigations http://lsda.jas.nasa.gov/books/apollo/s2CH2.htm
- Gardes M. Bruns T. D. 1993 ITS Primers With Enhanced Specificity for Basidiomycetes -Application to the Identification of Mycorrhizae and Rusts Molecular Ecology 2 113 118
- Kawamura Y. Li Y. Liu H. Huang X. Li Z. Ezaki T. 2001 Bacterial Population in Russian Space Station “Mir” Microbiol. Immunol. 45 819 828
- Klamer M. Roberts M. S. Levine L. H. Drake B. G. Garland J. L. 2002 Influence of Elevated Carbon Dioxide on the Fungal Community in a Coastal Scrub Oak Forest Soil Investigated Using Terminal Restriction Length Polymorphism Analysis Applied and Environmental Microbiology 68
- Lane D. J. 1991 16S/23S Sequencing Goodfellow M Nucleic Acid Techniques in Bacterial Systematics John Wiley & Sons, Inc. New York, NY 115 176
- Liu W.-T. Marsh T. L. Cheng H. Forney L. J. 1997 Characterization of Microbial Diversity by Determining Terminal Restriction Fragment Length Polymorphisms of Genes Encoding 16S rRNA Applied and Environmental Microbiology 63 4516 4522
- Makimura Koichi Ryo Hanazawa Kosuke Takatori Yoshiko Tamura Ryuichi Fujisaki Yayoi Nishiyama Shigeru Abe Katsuhisa Uchida Yoshiaki Kawamura Takayuki Ezaki Hideyo Yamaguchi 2001 Fungal Flora on Board the Mir-Space Station, Identification by Morphological Features Microbiol. Immunol. 45 357 363
- Novikova Natalja D. 2000 Survey of the Microbial Contamination Knowledge On Board Manned Russian Space Vehicles Final presentation to the European Space Agency http://industry.esa.int/ATTACHEMENTS/A114/novikova.pdf
- Ott Mark. 2002 Microbial Monitoring of the International Space Station: Current Operations and Goals for the Future Environmental Sentinals Symposium Johnson Space Center 17-18 September 2002
- Skuratov V. M. Zagibalova L. B. Pushkin V. P. 2002 Species Composition and Microbial Contamination of Atmospheric Humidity Condensate and Potable Water from the Mir Water Supply Systems During Mir Main Missions 4 Through 27 Aviakosm. Ekolog. Med 36 2 28 32
- Venkateswaran Kasthuri Satomi Masataka Chung Shirley Kern Roger Koukol Robert Basic Cecilia White David 2001 Molecular Microbial Diversity of a Spacecraft Assembly Facility Systematic and Applied Microbiology 24 311 320
- White T. J. Bruns T. Lee S. Taylor J. W. 1990 Amplification and Direct Sequencing of Fungal Ribosomal RNA Genes for Phylogenetics White T. J. PCR Protocols: A Guide to Methods and Applications Academic Press, Inc. New York, NY 315 322