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Toxicological Impact of Diesel Fuel Supplementation with Rapeseed Methylester (RME) on the Lung Toxic Potential of Diesel Engine Exhausts
Technical Paper
2000-01-2060
ISSN: 0148-7191, e-ISSN: 2688-3627
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English
Abstract
Organotypic cultures of precision-cut rat lung slices are exposed three hours to 5 dilutions of a continuous exhaust flow. The exhausts from three fuelings are tested (filtered and not): gazole, gazole/RME mixture (70 % / 30%) and RME.
Different parameters are assessed: ATP and GSH (cell viability), TNFα production (proinflammatory response) and nucleosomes (apoptosis). After exposure, the ATP level is only modified by the highest concentrations of gazole/RME or RME exhausts (filtered and not); the GSH level is decreased for each of the concentrated filtered exhausts. Moreover, only the whole gazole exhaust leads to a modification of TNFα production. At last, the whole gazole exhaust leads to an increase of slice nucleosome level in a dose-dependent way; RME supplementation or filtration significantly attenuates this effect.
In conclusion, whole diesel exhaust induces an inflammatory response and DNA alterations, which are partially or totally reversed by filtration and/or by RME supplementation.
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Authors
Citation
Le Prieur, E., Morin, J., Bion, A., Gouriou, F. et al., "Toxicological Impact of Diesel Fuel Supplementation with Rapeseed Methylester (RME) on the Lung Toxic Potential of Diesel Engine Exhausts," SAE Technical Paper 2000-01-2060, 2000, https://doi.org/10.4271/2000-01-2060.Also In
Particle Size Distribution in the Exhaust of Diesel and Gasoline Engines
Number: SP-1552; Published: 2000-06-19
Number: SP-1552; Published: 2000-06-19
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